College of Life Sciences, Southwest Forestry University, Kunming 650224, China.
Key Laboratory for Forest Genetic and Tree Improvement and Propagation in University of YunnanProvince, Southwest Forestry University, Kunming 650224, China.
Genes (Basel). 2024 Jan 23;15(2):148. doi: 10.3390/genes15020148.
(1) Background: Brassinosteroids (BRs) are important hormones involved in almost all stages of plant growth and development, and sterol dehydrogenase is a key enzyme involved in BRs biosynthesis. However, the sterol dehydrogenase gene family of Dode () has not been studied. (2) Methods: The () gene family was identified and analyzed. Three genes were screened based on RNA-seq of the stem tips, and the was further investigated via qRT-PCR (quantitative real-time polymerase chain reaction) and subcellular localization. (3) Results: The 14 family genes in were categorized into four groups, and 10 conserved protein motifs were identified. The gene structure, chromosome distribution, collinearity, and codon preference of all genes in the genome were analyzed. The codon preference of this family is towards the A/U ending, which is strongly influenced by natural selection. The gene was expressed at a higher level in September than in July, and it was significantly expressed in stems, stem tips, and leaves. The protein was localized in chloroplasts. (4) Conclusions: The plays an important role in the rapid growth period of . This systematic analysis provides a basis for the genome-wide identification of genes related to the brassinolide biosynthesis process in , and lays a foundation for the study of the rapid growth mechanism of .
(1) 背景：油菜素内酯（BRs）是参与植物生长发育几乎所有阶段的重要激素，甾醇脱氢酶是 BRs 生物合成中的关键酶。然而，油菜（）甾醇脱氢酶基因家族尚未得到研究。(2) 方法：鉴定和分析了 () 基因家族。基于茎尖的 RNA-seq 筛选了 3 个基因，并通过 qRT-PCR（定量实时聚合酶链反应）和亚细胞定位进一步研究了。(3) 结果：将 14 个 家族基因分为 4 组，鉴定出 10 个保守蛋白基序。分析了 基因组中所有 基因的基因结构、染色体分布、共线性和密码子偏好性。该家族的密码子偏好性倾向于 A/U 结尾，强烈受到自然选择的影响。基因在 9 月的表达水平高于 7 月，在茎、茎尖和叶片中表达显著。蛋白定位于叶绿体。(4) 结论：在 快速生长期间发挥重要作用。这项系统分析为油菜中与油菜内酯生物合成过程相关基因的全基因组鉴定提供了基础，并为研究的快速生长机制奠定了基础。
