Wang Shuai, Wang Yifan, Yao Youzhi, Li Wenzhuo, Hu Zhan, Li Dong, Sun Ranfeng
Key Laboratory of Green Prevention and Control of Tropical Plant Diseases and Pests, Ministry of Education, School of Tropical Agriculture and Forestry, Hainan University, Haikou 570228, China.
Int J Mol Sci. 2025 Apr 3;26(7):3335. doi: 10.3390/ijms26073335.
is an important pathogen causing bacterial wilt in pepper ( L.). The concurrent infection of and root-knot nematodes spp.) exacerbates the severity of bacterial wilt in pepper. Utilizing plant endophytic bacteria to control these mixed diseases is a viable strategy. L. (Sterculiaceae) is a traditional medicine plant. A total of 209 endophytic bacteria were isolated from , and W-126 showed an efficient antagonistic effect against . Based on active compound tracking principles, a compound was isolated through silica gel column chromatography and preparative HPLC combined with TLC analysis. It was identified as phenazine-1-carboxamide (PCN) by spectral techniques (ESI-MS, H-NMR, C-NMR). PCN displayed excellent inhibitory activity against , with an EC of 64.16 μg/mL in vitro. In addition, it showed certain nematocide activity, with an LC value of 118.63 μg/mL at 72 h. PCN also showed certain inhibitory activity against five other phytopathogenic bacteria. The structure-activity relationship indicated that the phenazine skeleton and acylamide groups were the key pharmacophores for the activity of phenazine-related compounds against . PCN controlled the complex diseases of and in a pot experiment, with respective 51.41 and 39.80% inhibitory rates. The exploration of secondary metabolites of biocontrol bacteria can provide reference for the development of novel and efficient pesticides.
是引起辣椒细菌性萎蔫病的重要病原菌。与根结线虫（南方根结线虫等）同时感染会加剧辣椒细菌性萎蔫病的严重程度。利用植物内生细菌控制这些混合病害是一种可行的策略。苹婆（梧桐科）是一种传统药用植物。从苹婆中总共分离出209株内生细菌，其中W-126对表现出高效的拮抗作用。基于活性化合物追踪原理，通过硅胶柱色谱、制备型高效液相色谱结合薄层色谱分析分离出一种化合物。通过光谱技术（电喷雾质谱、氢核磁共振、碳核磁共振）鉴定其为吩嗪-1-甲酰胺（PCN）。PCN在体外对表现出优异的抑制活性，其半数有效浓度为64.16μg/mL。此外，它在72小时时表现出一定的杀线虫活性，致死中浓度值为118.63μg/mL。PCN对其他五种植物病原菌也表现出一定的抑制活性。构效关系表明，吩嗪骨架和酰胺基团是吩嗪类化合物对活性的关键药效基团。在盆栽试验中，PCN对和的复合病害有防治作用，抑制率分别为51.41%和39.80%。对生防细菌次生代谢产物的探索可为新型高效农药的开发提供参考。
