Singh Pragya, Easley Acarizia, Menchaca Karla Tapia, Fanniel Victor, Gomez Raymond, Marquez Joanna, Hill Shauna
RegenTX Labs LLC, 3463 Magic Dr Ste 315, San Antonio, TX 78229, USA.
Int J Mol Sci. 2025 Apr 5;26(7):3406. doi: 10.3390/ijms26073406.
Human placental-derived allografts are biomaterials categorized as cellular, acellular, matrix-like products (CAMPs) that can serve as wound coverings due to placenta tissue's innate barrier function. The placental membrane consists of three layers, the amnion, the intermediate layer (IL), and the chorion, each contributing distinct functional and biological properties. This study investigates how variations in layer composition influence the Extracellular Matrix (ECM) and growth factor profiles of placental allografts. We compared Dual Layer (amnion-amnion), Full Thickness (amnion-intermediate-chorion, FT), and a novel four-layer allograft configuration (amnion-intermediate-chorion-amnion, ACA). Histological analyses using hematoxylin and eosin (H&E) and Masson's trichrome staining revealed distinct structural architecture among the three allografts, with FT and ACA exhibiting 4.9 times and 5.7 times greater thickness as compared with the Dual Layer, respectively. Compositional studies revealed different concentrations of key ECM components (collagen, elastin, proteoglycans, hyaluronic acid) and growth factors (ANG-2, EGF, PDGF-AA, VEGF) across allografts. The collagen concentration was two times higher in ACA as compared with the Dual Layer and FT. Additionally, FT and ACA demonstrated higher levels of growth factors and other ECM components, underscoring their biochemical diversity. These findings highlight the fact that the structural and biochemical properties of placental-derived allografts depend on their layer composition. This study underscores the importance of tailoring layer configurations that are optimized for clinical applications of CAMPs, enabling clinicians to select the most suitable grafts for clinical use, such as for wound management.
人胎盘来源的同种异体移植物是一类生物材料，可分为细胞型、无细胞型、基质样产品（CAMP），由于胎盘组织具有天然屏障功能，可作为伤口覆盖物。胎盘膜由三层组成，即羊膜、中间层（IL）和绒毛膜，每层都具有独特的功能和生物学特性。本研究调查了层组成的变化如何影响胎盘同种异体移植物的细胞外基质（ECM）和生长因子谱。我们比较了双层（羊膜-羊膜）、全层（羊膜-中间层-绒毛膜，FT）和一种新型的四层同种异体移植物结构（羊膜-中间层-绒毛膜-羊膜，ACA）。使用苏木精和伊红（H&E）以及马松三色染色的组织学分析显示，三种同种异体移植物之间具有明显不同的结构架构，与双层相比，FT和ACA的厚度分别大4.9倍和5.7倍。成分研究揭示了同种异体移植物中关键ECM成分（胶原蛋白、弹性蛋白、蛋白聚糖、透明质酸）和生长因子（ANG-2、EGF、PDGF-AA、VEGF）的浓度不同。与双层和FT相比，ACA中的胶原蛋白浓度高出两倍。此外，FT和ACA显示出更高水平的生长因子和其他ECM成分，突出了它们的生化多样性。这些发现凸显了胎盘来源的同种异体移植物的结构和生化特性取决于其层组成这一事实。本研究强调了定制针对CAMP临床应用进行优化的层配置的重要性，使临床医生能够选择最适合临床使用的移植物，例如用于伤口管理。
