Hatori Sena, Yamaguchi Sho T, Kobayashi Riho, Okamoto Kazuki, Zhou Zhiwen, Kotake Koki T, Matsui Futaba, Hioki Hiroyuki, Norimoto Hiroaki
Laboratory for Neuroethology, Graduate School of Science, Nagoya University, Nagoya 464-8602, Japan.
Department of Cellular Pharmacology, Graduate School of Medicine, Hokkaido University, Sapporo 060-8638, Japan.
Proc Natl Acad Sci U S A. 2025 Apr 22;122(16):e2415929122. doi: 10.1073/pnas.2415929122. Epub 2025 Apr 17.
Slow-wave sleep (SWS) and rapid eye movement sleep are the two primary components of electrophysiological sleep (e-sleep) in mammals and birds. Slow waves in the cortex not only characterize SWS but are also used as biological markers for sleep homeostasis, given their rebound after sleep deprivation (SD). Recently, it has been reported that the Australian dragon exhibits a two-stage sleep pattern in the dorsal ventricular ridge (DVR), which includes a homologue of the mammalian claustrum (CLA). It remains unclear whether reptilian e-sleep, which has been characterized by activity outside the cortex, compensates for sleep loss, as observed in mammals. We here report a significant rebound in the local field potential (LFP) after 7 h of SD. Meanwhile, the mean bout length of each sleep state remained unaffected. We further investigated a possible role of the cortex in e-sleep regulation and homeostasis in and found that although a corticotomy had no obvious effect on the LFP features of baseline sleep, it abolished LFP power rebound in the CLA/DVR after SD. These findings suggest that e-sleep homeostasis is a common feature in amniotes and that the cortex is involved in regulating activity rebounds in reptiles and mammals.
慢波睡眠（SWS）和快速眼动睡眠是哺乳动物和鸟类电生理睡眠（e睡眠）的两个主要组成部分。皮层中的慢波不仅是慢波睡眠的特征，还因其在睡眠剥夺（SD）后的反弹而被用作睡眠稳态的生物学标志物。最近，有报道称澳大利亚鬃狮蜥在背侧室嵴（DVR）呈现两阶段睡眠模式，其中包括与哺乳动物屏状核（CLA）同源的结构。目前尚不清楚以皮层外活动为特征的爬行动物e睡眠是否像在哺乳动物中观察到的那样能补偿睡眠缺失。我们在此报告，睡眠剥夺7小时后局部场电位（LFP）出现显著反弹。同时，每种睡眠状态的平均发作时长保持不变。我们进一步研究了皮层在e睡眠调节和稳态中的可能作用，发现虽然皮层切开术对基线睡眠的LFP特征没有明显影响，但它消除了睡眠剥夺后屏状核/背侧室嵴中的LFP功率反弹。这些发现表明，e睡眠稳态是羊膜动物的一个共同特征，并且皮层参与调节爬行动物和哺乳动物的活动反弹。
