• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

健康状态以及埃博拉和尼帕病毒感染期间人类和灵长类动物组织中的细胞免疫表型分析。

Cellular immunophenotyping in human and primate tissues during healthy conditions and Ebola and Nipah infections.

作者信息

Platt Andrew P, Barr Bobbi, Marketon Anthony, Bernbaum Rebecca, Rivera Deja Fp, Munster Vincent J, Chertow Daniel S, Holbrook Michael R, Anthony Scott M, Pahar Bapi

机构信息

Laboratory of Virology, National Institute of Allergy and Infectious Diseases.

Emerging Pathogens Section, Critical Care Medicine Department, Clinical Center, and.

出版信息

JCI Insight. 2025 Apr 17;10(11). doi: 10.1172/jci.insight.185861. eCollection 2025 Jun 9.

DOI:10.1172/jci.insight.185861
PMID:40244690
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12220940/
Abstract

We developed a 29-color spectral cytometry panel to enhance nonhuman primate (NHP) models for cross-reactive immunophenotyping. This panel is suitable for biosafety level 4 (BSL-4) viruses and can be used with both human and NHP samples in BSL-2 research settings. Tissues from humans, rhesus monkeys (RhMs), crab-eating macaques (CEMs), and green monkeys (GMs) were stained with a 29-color immunophenotyping panel requiring only 2 clone substitutions. Comparable staining was observed for all samples. Unbiased analysis showed acceptable overlap in T cell phenotypes across samples, with differences in human and NHP B cells and granulocytes. In CEMs, most circulating CD8+ T cells were from effector memory cells, with significantly higher levels than in humans, RhMs, and GMs. Analysis of samples from various anatomical sites revealed distinct location-specific phenotypes. In Nipah virus-exposed animals, splenocytes showed a substantial increase in IgM+ B cells and a reduction in effector memory CD8+ T cells compared with unexposed controls. Lymph nodes from Ebola virus-exposed animals showed a loss of CXCR3+CD8+ T cells versus unexposed controls. This panel may guide the development of additional multicolor panels in preclinical and clinical settings and may increase understanding of the pathogenesis of diseases caused by emerging and reemerging viruses.

摘要

我们开发了一种29色光谱细胞术检测板，以增强非人类灵长类动物（NHP）模型用于交叉反应免疫表型分析。该检测板适用于生物安全4级（BSL-4）病毒，并且可在BSL-2研究环境中用于人类和NHP样本。来自人类、恒河猴（RhM）、食蟹猕猴（CEM）和绿猴（GM）的组织用仅需2个克隆替换的29色免疫表型检测板进行染色。所有样本均观察到可比的染色情况。无偏分析显示不同样本间T细胞表型存在可接受的重叠，而人类和NHP的B细胞及粒细胞存在差异。在食蟹猕猴中，大多数循环CD8+ T细胞来自效应记忆细胞，其水平显著高于人类、恒河猴和绿猴。对来自不同解剖部位的样本分析揭示了不同的部位特异性表型。在感染尼帕病毒的动物中，与未感染对照相比，脾细胞中IgM+ B细胞大幅增加，效应记忆CD8+ T细胞减少。与未感染对照相比，感染埃博拉病毒动物的淋巴结中CXCR3+CD8+ T细胞减少。该检测板可能会指导临床前和临床环境中其他多色检测板的开发，并可能增进对由新出现和重新出现的病毒引起的疾病发病机制的理解。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/07e0/12220940/871946c41f16/jciinsight-10-185861-g186.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/07e0/12220940/81613e234040/jciinsight-10-185861-g183.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/07e0/12220940/99e63a20b21c/jciinsight-10-185861-g184.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/07e0/12220940/7c861097336f/jciinsight-10-185861-g185.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/07e0/12220940/871946c41f16/jciinsight-10-185861-g186.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/07e0/12220940/81613e234040/jciinsight-10-185861-g183.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/07e0/12220940/99e63a20b21c/jciinsight-10-185861-g184.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/07e0/12220940/7c861097336f/jciinsight-10-185861-g185.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/07e0/12220940/871946c41f16/jciinsight-10-185861-g186.jpg

相似文献

1
Cellular immunophenotyping in human and primate tissues during healthy conditions and Ebola and Nipah infections.健康状态以及埃博拉和尼帕病毒感染期间人类和灵长类动物组织中的细胞免疫表型分析。
JCI Insight. 2025 Apr 17;10(11). doi: 10.1172/jci.insight.185861. eCollection 2025 Jun 9.
2
Signs and symptoms to determine if a patient presenting in primary care or hospital outpatient settings has COVID-19.在基层医疗机构或医院门诊环境中，如果患者出现以下症状和体征，可判断其是否患有 COVID-19。
Cochrane Database Syst Rev. 2022 May 20;5(5):CD013665. doi: 10.1002/14651858.CD013665.pub3.
3
Antibody tests for identification of current and past infection with SARS-CoV-2.抗体检测用于鉴定 SARS-CoV-2 的现症感染和既往感染。
Cochrane Database Syst Rev. 2022 Nov 17;11(11):CD013652. doi: 10.1002/14651858.CD013652.pub2.
4
Systemic treatments for metastatic cutaneous melanoma.转移性皮肤黑色素瘤的全身治疗
Cochrane Database Syst Rev. 2018 Feb 6;2(2):CD011123. doi: 10.1002/14651858.CD011123.pub2.
5
Systemic pharmacological treatments for chronic plaque psoriasis: a network meta-analysis.系统性药理学治疗慢性斑块状银屑病：网络荟萃分析。
Cochrane Database Syst Rev. 2021 Apr 19;4(4):CD011535. doi: 10.1002/14651858.CD011535.pub4.
6
Immunogenicity and seroefficacy of pneumococcal conjugate vaccines: a systematic review and network meta-analysis.肺炎球菌结合疫苗的免疫原性和血清效力：系统评价和网络荟萃分析。
Health Technol Assess. 2024 Jul;28(34):1-109. doi: 10.3310/YWHA3079.
7
Personal protective equipment for preventing highly infectious diseases due to exposure to contaminated body fluids in healthcare staff.用于预防医护人员因接触受污染体液而感染高传染性疾病的个人防护装备。
Cochrane Database Syst Rev. 2016 Apr 19;4:CD011621. doi: 10.1002/14651858.CD011621.pub2.
8
Physical interventions to interrupt or reduce the spread of respiratory viruses.物理干预措施以阻断或减少呼吸道病毒的传播。
Cochrane Database Syst Rev. 2023 Jan 30;1(1):CD006207. doi: 10.1002/14651858.CD006207.pub6.
9
Systemic pharmacological treatments for chronic plaque psoriasis: a network meta-analysis.系统性药理学治疗慢性斑块型银屑病：网络荟萃分析。
Cochrane Database Syst Rev. 2022 May 23;5(5):CD011535. doi: 10.1002/14651858.CD011535.pub5.
10
Intraoperative frozen section analysis for the diagnosis of early stage ovarian cancer in suspicious pelvic masses.术中冰冻切片分析用于诊断可疑盆腔肿块中的早期卵巢癌。
Cochrane Database Syst Rev. 2016 Mar 1;3(3):CD010360. doi: 10.1002/14651858.CD010360.pub2.

本文引用的文献

1
Malaysia outbreak survivors retain detectable Nipah antibodies and memory B cells after 25 years.马来西亚疫情幸存者在25年后仍可检测到尼帕抗体和记忆B细胞。
J Infect. 2025 Feb;90(2):106398. doi: 10.1016/j.jinf.2024.106398. Epub 2025 Jan 3.
2
Age and sex associated organ weight differences in vervets/African green monkeys (Chlorocebus aethiops sabaeus).黑长尾猴/非洲绿猴（埃塞俄比亚绿猴亚种）中与年龄和性别相关的器官重量差异
J Med Primatol. 2024 Aug;53(4):e12721. doi: 10.1111/jmp.12721.
3
Case fatality rate for Ebola disease, 1976-2022: A meta-analysis of global data.
1976-2022 年埃博拉病病死率的荟萃分析：全球数据。
J Infect Public Health. 2024 Jan;17(1):25-34. doi: 10.1016/j.jiph.2023.10.020. Epub 2023 Oct 27.
4
Nipah virus: India's Kerala state moves quickly to control fresh outbreak.尼帕病毒：印度喀拉拉邦迅速采取行动控制新疫情。
BMJ. 2023 Sep 15;382:2117. doi: 10.1136/bmj.p2117.
5
Depletion of Bone Marrow Hematopoietic Cells in Ebolavirus-Infected Rhesus Macaques: A Possible Cause of Hematologic Abnormalities in Ebolavirus Disease.埃博拉病毒感染恒河猴骨髓造血细胞耗竭：埃博拉病毒病血液学异常的可能原因。
Am J Pathol. 2023 Dec;193(12):2031-2046. doi: 10.1016/j.ajpath.2023.08.010. Epub 2023 Sep 7.
6
Clinical and Immunologic Correlates of Vasodilatory Shock Among Ebola Virus-Infected Nonhuman Primates in a Critical Care Model.在重症监护模型中，埃博拉病毒感染的非人类灵长类动物中血管扩张性休克的临床和免疫相关性。
J Infect Dis. 2023 Nov 13;228(Suppl 7):S635-S647. doi: 10.1093/infdis/jiad374.
7
Ebola Virus Disease Features Hemophagocytic Lymphohistiocytosis/Macrophage Activation Syndrome in the Rhesus Macaque Model.埃博拉病毒病在恒河猴模型中具有噬血细胞性淋巴组织细胞增多症/巨噬细胞活化综合征的特征。
J Infect Dis. 2023 Aug 16;228(4):371-382. doi: 10.1093/infdis/jiad203.
8
Recombinant Simian Varicella Virus-Simian Immunodeficiency Virus Vaccine Induces T and B Cell Functions and Provides Partial Protection against Repeated Mucosal SIV Challenges in Rhesus Macaques.重组猴痘病毒-猴免疫缺陷病毒疫苗可诱导 T 和 B 细胞功能，并为恒河猴反复黏膜感染猴免疫缺陷病毒提供部分保护。
Viruses. 2022 Dec 17;14(12):2819. doi: 10.3390/v14122819.
9
mTOR regulates T cell exhaustion and PD-1-targeted immunotherapy response during chronic viral infection.mTOR 调控慢性病毒感染期间 T 细胞耗竭和 PD-1 靶向免疫治疗反应。
J Clin Invest. 2023 Jan 17;133(2):e160025. doi: 10.1172/JCI160025.
10
Development of a Well-Characterized Cynomolgus Macaque Model of Marburg Virus Disease for Support of Vaccine and Therapy Development.开发一种特征明确的马尔堡病毒病食蟹猴模型以支持疫苗和疗法开发。
Vaccines (Basel). 2022 Aug 14;10(8):1314. doi: 10.3390/vaccines10081314.