Lun Jianchi, Liu Mengjie, Zhang Wenbo, Huang Gengxiong, Ma Ming, Jin Wenxin, Zhu Yongqi, Qu Qian, Lv Weijie, Guo Shining
College of Veterinary Medicine, South China Agricultural University, Guangzhou, PRC.
College of Veterinary Medicine, South China Agricultural University, Guangzhou, PRC; School of Animal Science and Technology, Foshan University, Foshan, PRC.
Poult Sci. 2025 Mar 29;104(6):105106. doi: 10.1016/j.psj.2025.105106.
This study focused on examining how Portulaca oleracea L. extract (POLE) influences production performance, antioxidant capacity, immunity, anti-apoptotic ability, and intestinal health in chicks exposed to Escherichia coli O78(E. coli). A 2 × 2 factorial design was used to randomly divide male chicks into 4 groups: (1) basal diet (CON group), (2) basal diet supplemented with 0.5 g/kg POL (CPOL group), (3) basal diet with E. coli challenge (ECON group), and (4) basal diet supplemented with 0.5 g/kg POL as well as E. coli challenge (EPOL group).The experiment lasted for 19 days. The POL supplementation was found to decrease the feed conversion ratio and liver and spleen indexes and increase the average daily gain and bursa indexes (P<0.05). Moreover, in chicks infected with E. coli, POL reduced the levels of serum DAO and caspase 8 (P<0.05). POL notably elevated the serum levels of immunoglobulins and IL-10, an anti-inflammatory cytokine, which also decreased the levels of proinflammatory cytokines TNF-α and IFN-γ. At the same time, dietary POL enhanced the mRNA expression of genes related to antioxidation and reduced the expression of genes associated with proinflammatory cytokines and apoptosis in the liver, spleen, jejunum, and ileum. In addition, dietary POL repaired the intestinal barrier and injury. More importantly, by boosting the abundance of beneficial bacteria such as Butyricicoccus and Blautia, and reducing harmful bacteria like Enterococcus and Escherichia, POL induced changes in the gut microbiota composition. Spearman's correlation study revealed that Dorea and Butyricoccus had a strong positive link with intestinal barrier function and antioxidant capacity, while Escherichia had a substantial positive correlation with pro-inflammatory cytokines. In summary, this study suggests adding POL to the diet as a beneficial supplement to enhance chick performance.
本研究聚焦于考察马齿苋提取物（POLE）对感染大肠杆菌O78的雏鸡生产性能、抗氧化能力、免疫力、抗凋亡能力及肠道健康的影响。采用2×2析因设计将雄性雏鸡随机分为4组：（1）基础日粮组（CON组），（2）基础日粮添加0.5 g/kg POL组（CPOL组），（3）基础日粮+大肠杆菌攻毒组（ECON组），（4）基础日粮添加0.5 g/kg POL且大肠杆菌攻毒组（EPOL组）。实验持续19天。结果发现，添加POL可降低料重比以及肝脏和脾脏指数，并提高平均日增重和法氏囊指数（P<0.05）。此外，在感染大肠杆菌的雏鸡中，POL降低了血清DAO和半胱天冬酶8水平（P<0.05）。POL显著提高了血清免疫球蛋白和抗炎细胞因子IL-10的水平，同时降低了促炎细胞因子TNF-α和IFN-γ的水平。同时，日粮POL增强了肝脏、脾脏、空肠和回肠中与抗氧化相关基因的mRNA表达，并降低了与促炎细胞因子和凋亡相关基因的表达。此外，日粮POL修复了肠道屏障和损伤。更重要的是，通过增加丁酸球菌属和布劳特氏菌属等有益菌的丰度，并减少肠球菌属和大肠杆菌等有害菌，POL诱导了肠道微生物群组成的变化。Spearman相关性研究表明，瘤胃球菌属和丁酸球菌属与肠道屏障功能和抗氧化能力呈强正相关，而大肠杆菌与促炎细胞因子呈显著正相关。总之，本研究表明在日粮中添加POL作为有益补充剂可提高雏鸡性能。
