Saito K, Yamazoe Y, Kamataki T, Kato R
Xenobiotica. 1985 Apr;15(4):327-32. doi: 10.3109/00498258509045367.
Thermally labile N-hydroxyarylamines, which are the active metabolites of carcinogenic/mutagenic aromatic amines and show potent direct mutagenicity, were studied by fast-atom bombardment (F.A.B.) mass spectrometry and electron-impact (E.I.) mass spectrometry. The protonated molecular ion [M + H]+ and the molecular ion [M]+ were observed at high intensity in the F.A.B. mode. The fragment ions corresponding to [M + H-16]+, [M + H-17]+ and [M-16]+, [M-17]+, [M + H-32]+ and [M-32]+ were also observed characteristically. The quasimolecular ion peaks were shifted up by the numbers of active hydrogens in molecules after the hydrogen-deuterium exchange with [hydroxy--2H3]glycerol and 2H2O. The formation of the ions continued stably throughout the period of measurement, and the decomposition of the samples did not occur in the F.A.B. ion source, compared with the E.I. mode. Hence, it is suggested that the F.A.B. technique is useful for the analysis of the heat-labile toxicologically important N-hydroxyarylamines.
热不稳定的N-羟基芳胺是致癌/致突变性芳香胺的活性代谢产物，具有很强的直接致突变性，本文采用快原子轰击（F.A.B.）质谱法和电子轰击（E.I.）质谱法对其进行了研究。在F.A.B.模式下，观察到质子化分子离子[M + H]+和分子离子[M]+的高强度信号。还特征性地观察到对应于[M + H-16]+、[M + H-17]+以及[M-16]+、[M-17]+、[M + H-32]+和[M-32]+的碎片离子。在用[羟基-2H3]甘油和2H2O进行氢-氘交换后，准分子离子峰根据分子中活性氢的数量向上移动。与E.I.模式相比，在整个测量期间离子的形成持续稳定，且样品在F.A.B.离子源中未发生分解。因此，表明F.A.B.技术对于分析热不稳定的具有重要毒理学意义的N-羟基芳胺是有用的。
