Sethi S K, Smith D L, McCloskey J A
Biochem Biophys Res Commun. 1983 Apr 15;112(1):126-31. doi: 10.1016/0006-291x(83)91806-5.
Fast atom bombardment mass spectrometry following hydrogen--deuterium exchange in [hydroxy-2H3]glycerol and 2H20 has been studied as a means of establishing active hydrogen content in molecules of unknown structure. Nucleotides, carbohydrates, one peptide and complex antibiotics, in the mass region to 1500 daltons and 29 exchangeable hydrogens were examined, with a correct hydrogen count unambiguously measured in every case. The method is experimentally simple and applicable on a microgram scale, to salts and a variety of polar compounds of biological origin.
对[羟基-2H₃]甘油和2H₂O中氢-氘交换后的快原子轰击质谱法进行了研究，以此作为确定未知结构分子中活性氢含量的一种手段。研究了分子量在1500道尔顿以下、含有29个可交换氢的核苷酸、碳水化合物、一种肽和复杂抗生素，在每种情况下都能明确无误地准确测定氢的数量。该方法实验操作简单，适用于微克级规模，可用于盐类以及多种生物来源的极性化合物。
