Misle J A, Márquez M E, Hernández A G
Z Parasitenkd. 1985;71(4):419-28. doi: 10.1007/BF00928344.
A simple procedure that allowed the extraction and partial purification of a component of 65,000 mol.wt. from the surface of Leishmania braziliensis promastigotes is described. Iodinated cell surface membrane fractions were solubilized using Triton X-100 followed by Nonidet P-40. The macromolecular components were then freed of the detergents by passage of the extracts through a column of DE52 cellulose. The component of 65,000 mol.wt. was eluted from the column with 1 m NaCl. This component in whole parasites was immunoprecipitated by sera from patients with cutaneous, mucocutaneous, and diffuse cutaneous leishmaniasis and kala-azar. None of the major surface determinants reacted with sera from normal individuals with antibovine rabbit serum. The relevance and the possible applications on the immunoprophylaxis of the disease are briefly discussed.
本文描述了一种简单的方法，可从巴西利什曼原虫前鞭毛体表面提取并部分纯化一种分子量为65,000的成分。用Triton X-100然后用Nonidet P-40溶解碘化的细胞表面膜部分。然后通过将提取物通过DE52纤维素柱使大分子成分去除去污剂。用1m NaCl从柱上洗脱分子量为65,000的成分。皮肤、黏膜皮肤和弥漫性皮肤利什曼病以及黑热病患者的血清可免疫沉淀全寄生虫中的该成分。正常个体的血清与抗牛兔血清均未与任何主要表面决定簇发生反应。本文简要讨论了该成分与疾病免疫预防的相关性及可能的应用。
