• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

磷酸核糖焦磷酸合成酶作为代谢阀促进了甲基杆菌/甲基单胞菌叶际定殖和植物生长。

Phosphoribosylpyrophosphate synthetase as a metabolic valve advances Methylobacterium/Methylorubrum phyllosphere colonization and plant growth.

机构信息

School of Life Sciences, Qingdao Agricultural University, Qingdao, Shandong, PR China.

Shandong Province Key Laboratory of Applied Mycology, Qingdao Agricultural University, Qingdao, Shandong, PR China.

出版信息

Nat Commun. 2024 Jul 16;15(1):5969. doi: 10.1038/s41467-024-50342-9.

DOI:10.1038/s41467-024-50342-9
PMID:39013920
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11252147/
Abstract

The proficiency of phyllosphere microbiomes in efficiently utilizing plant-provided nutrients is pivotal for their successful colonization of plants. The methylotrophic capabilities of Methylobacterium/Methylorubrum play a crucial role in this process. However, the precise mechanisms facilitating efficient colonization remain elusive. In the present study, we investigate the significance of methanol assimilation in shaping the success of mutualistic relationships between methylotrophs and plants. A set of strains originating from Methylorubrum extorquens AM1 are subjected to evolutionary pressures to thrive under low methanol conditions. A mutation in the phosphoribosylpyrophosphate synthetase gene is identified, which converts it into a metabolic valve. This valve redirects limited C1-carbon resources towards the synthesis of biomass by up-regulating a non-essential phosphoketolase pathway. These newly acquired bacterial traits demonstrate superior colonization capabilities, even at low abundance, leading to increased growth of inoculated plants. This function is prevalent in Methylobacterium/Methylorubrum strains. In summary, our findings offer insights that could guide the selection of Methylobacterium/Methylorubrum strains for advantageous agricultural applications.

摘要

叶片微生物组在有效利用植物提供的养分方面的能力对于它们成功定殖植物至关重要。甲基营养菌（Methylobacterium/Methylorubrum）的甲醇营养能力在这个过程中起着关键作用。然而，促进有效定殖的精确机制仍不清楚。在本研究中，我们研究了甲醇同化在塑造甲基营养菌和植物之间互利关系的成功中的重要性。一组源自 Methylorubrum extorquens AM1 的菌株受到进化压力的影响，以在低甲醇条件下茁壮成长。鉴定出磷酸核糖焦磷酸合成酶基因发生突变，将其转化为代谢阀。该阀通过上调非必需的磷酸酮解酶途径将有限的 C1 碳资源重新导向生物量的合成。这些新获得的细菌特性表现出优越的定殖能力，即使在低丰度下，也能导致接种植物的生长增加。这种功能在 Methylobacterium/Methylorubrum 菌株中很普遍。总之，我们的研究结果提供了可以指导选择 Methylobacterium/Methylorubrum 菌株用于有利的农业应用的见解。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6b87/11252147/6b0fcb05a90b/41467_2024_50342_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6b87/11252147/3ee726f08c2b/41467_2024_50342_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6b87/11252147/a2801837eed6/41467_2024_50342_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6b87/11252147/06e393a6a2ee/41467_2024_50342_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6b87/11252147/12b7a07607ea/41467_2024_50342_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6b87/11252147/fed20b681bed/41467_2024_50342_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6b87/11252147/6b0fcb05a90b/41467_2024_50342_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6b87/11252147/3ee726f08c2b/41467_2024_50342_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6b87/11252147/a2801837eed6/41467_2024_50342_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6b87/11252147/06e393a6a2ee/41467_2024_50342_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6b87/11252147/12b7a07607ea/41467_2024_50342_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6b87/11252147/fed20b681bed/41467_2024_50342_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6b87/11252147/6b0fcb05a90b/41467_2024_50342_Fig6_HTML.jpg

相似文献

1
Phosphoribosylpyrophosphate synthetase as a metabolic valve advances Methylobacterium/Methylorubrum phyllosphere colonization and plant growth.磷酸核糖焦磷酸合成酶作为代谢阀促进了甲基杆菌/甲基单胞菌叶际定殖和植物生长。
Nat Commun. 2024 Jul 16;15(1):5969. doi: 10.1038/s41467-024-50342-9.
2
Methylotrophic metabolism is advantageous for Methylobacterium extorquens during colonization of Medicago truncatula under competitive conditions.在竞争条件下，甲基营养型代谢对豌豆根瘤菌在蒺藜苜蓿定殖过程中具有优势。
Appl Environ Microbiol. 2005 Nov;71(11):7245-52. doi: 10.1128/AEM.71.11.7245-7252.2005.
3
Glycine betaine metabolism is enabled in PA1 by alterations to dimethylglycine dehydrogenase.通过对二甲基甘氨酸脱氢酶的改造，PA1中实现了甘氨酸甜菜碱代谢。
Appl Environ Microbiol. 2024 Jul 24;90(7):e0209023. doi: 10.1128/aem.02090-23. Epub 2024 Mar 27.
4
Functional investigation of methanol dehydrogenase-like protein XoxF in Methylobacterium extorquens AM1.甲醇脱氢酶样蛋白 XoxF 在甲基杆菌 AM1 中的功能研究。
Microbiology (Reading). 2010 Aug;156(Pt 8):2575-2586. doi: 10.1099/mic.0.038570-0. Epub 2010 May 6.
5
XoxF is required for expression of methanol dehydrogenase in Methylobacterium extorquens AM1.XoxF 对于甲基杆菌 AM1 中甲醇脱氢酶的表达是必需的。
J Bacteriol. 2011 Nov;193(21):6032-8. doi: 10.1128/JB.05367-11. Epub 2011 Aug 26.
6
Poly-3-hydroxybutyrate production in acetate minimal medium using engineered Methylorubrum extorquens AM1.利用工程化的嗜甲基红球菌AM1在乙酸盐基本培养基中生产聚-3-羟基丁酸酯。
Bioresour Technol. 2022 Jun;353:127127. doi: 10.1016/j.biortech.2022.127127. Epub 2022 Apr 7.
7
Difference in C3-C4 metabolism underlies tradeoff between growth rate and biomass yield in Methylobacterium extorquens AM1.甲基营养型芽孢杆菌AM1中C3-C4代谢差异是其生长速率与生物量产量之间权衡的基础。
BMC Microbiol. 2016 Jul 19;16(1):156. doi: 10.1186/s12866-016-0778-4.
8
Dichloromethane metabolism and C1 utilization genes in Methylobacterium strains.甲基杆菌菌株中的二氯甲烷代谢与C1利用基因。
Microbiology (Reading). 2002 Jun;148(Pt 6):1915-1922. doi: 10.1099/00221287-148-6-1915.
9
Production of 3-hydroxypropionic acid in engineered Methylobacterium extorquens AM1 and its reassimilation through a reductive route.工程化甲基杆菌 AM1 中 3-羟基丙酸的生产及其通过还原途径的再同化。
Microb Cell Fact. 2017 Oct 30;16(1):179. doi: 10.1186/s12934-017-0798-2.
10
Replacing the Ethylmalonyl-CoA Pathway with the Glyoxylate Shunt Provides Metabolic Flexibility in the Central Carbon Metabolism of Methylobacterium extorquens AM1.用乙醛酸循环替代乙基丙二酰辅酶A途径为甲基营养型甲基杆菌AM1的中心碳代谢提供了代谢灵活性。
ACS Synth Biol. 2018 Jan 19;7(1):86-97. doi: 10.1021/acssynbio.7b00229. Epub 2017 Dec 21.

引用本文的文献

1
Weather parameters and biotic factors synergistically shape the phyllosphere microbiome of pomelo ( (Burm.) Merr.) across annual cycle.气象参数和生物因子在全年周期中协同塑造了柚子（柚（Burm.）Merr.）的叶际微生物群落。
Front Plant Sci. 2025 Apr 3;16:1532188. doi: 10.3389/fpls.2025.1532188. eCollection 2025.
2
Temporal dynamics of walnut phyllosphere microbiota under synergistic pathogen exposure and environmental perturbation.协同病原体暴露和环境扰动下核桃叶际微生物群的时间动态
Front Microbiol. 2025 Apr 1;16:1551476. doi: 10.3389/fmicb.2025.1551476. eCollection 2025.
3
Transgenic Maize of Shapes Microbiome on Adaxial and Abaxial Surface of Leaves to Promote Disease Resistance.

本文引用的文献

1
Engineering photo-methylotrophic Methylobacterium for enhanced 3-hydroxypropionic acid production during non-growth stage fermentation.工程化光养甲基杆菌以增强非生长阶段发酵中的 3-羟基丙酸生产。
Bioresour Technol. 2024 Feb;393:130104. doi: 10.1016/j.biortech.2023.130104. Epub 2023 Nov 24.
2
Deep discovery informs difficult deployment in plant microbiome science.深度发现为植物微生物组科学的艰难部署提供了信息。
Cell. 2023 Oct 12;186(21):4496-4513. doi: 10.1016/j.cell.2023.08.035.
3
Characterization of C carotenoid and identification of its biosynthetic gene cluster in AM1.
转基因玉米塑造叶片正反表面的微生物群落以促进抗病性。
Microorganisms. 2025 Feb 7;13(2):362. doi: 10.3390/microorganisms13020362.
AM1中C类胡萝卜素的表征及其生物合成基因簇的鉴定。
Synth Syst Biotechnol. 2023 Aug 9;8(3):527-535. doi: 10.1016/j.synbio.2023.08.002. eCollection 2023 Sep.
4
A methanotrophic bacterium to enable methane removal for climate mitigation.一种能够去除甲烷以缓解气候变化的产甲烷菌。
Proc Natl Acad Sci U S A. 2023 Aug 29;120(35):e2310046120. doi: 10.1073/pnas.2310046120. Epub 2023 Aug 21.
5
Isolation and Characterization of Homologically Expressed Methanol Dehydrogenase from AM1 for the Development of Bioelectrocatalytical Systems.同源表达甲醇脱氢酶 AM1 的分离与表征及其在生物电化学系统中的应用。
Int J Mol Sci. 2022 Sep 7;23(18):10337. doi: 10.3390/ijms231810337.
6
How plants recruit their microbiome? New insights into beneficial interactions.植物如何招募其微生物组？有益相互作用的新见解。
J Adv Res. 2022 Sep;40:45-58. doi: 10.1016/j.jare.2021.11.020. Epub 2021 Dec 4.
7
Generation of an Escherichia coli strain growing on methanol via the ribulose monophosphate cycle.通过核酮糖单磷酸循环生成能够利用甲醇生长的大肠杆菌菌株。
Nat Commun. 2022 Sep 6;13(1):5243. doi: 10.1038/s41467-022-32744-9.
8
Dynamic character displacement among a pair of bacterial phyllosphere commensals in situ.一对定殖于植物叶片的细菌共生体的原位动态特征替代。
Nat Commun. 2022 May 20;13(1):2836. doi: 10.1038/s41467-022-30469-3.
9
Comprehensive Comparative Genomics and Phenotyping of Species.物种的综合比较基因组学与表型分析
Front Microbiol. 2021 Oct 6;12:740610. doi: 10.3389/fmicb.2021.740610. eCollection 2021.
10
Strategies for optimizing acetyl-CoA formation from glucose in bacteria.从葡萄糖优化细菌中乙酰辅酶 A 形成的策略。
Trends Biotechnol. 2022 Feb;40(2):149-165. doi: 10.1016/j.tibtech.2021.04.004. Epub 2021 May 5.