Sindhumati Jayaraman, Karpagaselvi Sanjai, Jayalakshmi Kumaraswamy, Lokesh Papaiah, Roopavathi Keshavaiah, Bhavna Pandey
Department of Oral Pathology and Microbiology, Rajiv Gandhi College of Dental Sciences and Research Centre, Cholanagar, Hebbal, R T Nagar Post, Bengaluru, Karnataka, India.
Department of Oral Pathology and Microbiology, Vydehi Institute of Dental Sciences and Research Centre, Nallurahalli, Whitefield, Bengaluru, Karnataka, India.
J Oral Maxillofac Pathol. 2025 Jan-Mar;29(1):35-40. doi: 10.4103/jomfp.jomfp_479_23. Epub 2025 Mar 28.
Mast cells are large granular cells that arise from multipotent CD 34+ precursors in the bone marrow normally distributed throughout the connective tissues. Following activation of immunologic or nonimmunologic stimuli, mast cells release secretory granules which give the characteristic metachromatic appearance with toluidine blue stain. Release of numerous mediators on degranulation of mast cells plays an important role in the pathogenesis of odontogenic cysts.
Odontogenic cysts, such as dentigerous cysts, arise due to the accumulation of fluid between the crown of an unerupted tooth and the reduced enamel epithelium. Dental follicles, which surround developing teeth, can also undergo cystic transformation. Mast cells activity might contribute to cyst expansion and bone resorption, highlighting their potential role in cystic pathology.
To study the presence of mast cells in the dental follicle and dentigerous cyst. To quantify the mast cells in the abovementioned subjects. To study the pattern of distribution of mast cell distribution in different zones of the study groups.
This was histopathological study conducted at the Department of Oral Pathology and Microbiology, Vydehi College of Dental Sciences and Research Centre, Bengaluru, between 2012 and 2015.
Our study was conducted in the Department of Oral Pathology and Microbiology at Vydehi College of Dental Sciences and Research Centre in the year 2012 to 2015. Histopathologically analyzed 30 cases each of dental follicle, and dentigerous cysts were taken and 4-5 micron sections were stained with toluidine blue. Counting of mast cells was done in three different zones which included subepithelial, intermediate, and deep zone. The results were tabulated and statistically analyzed.
Kruskal-Wallis Chi-squared test.
Both dental follicles and dentigerous cysts showed the presence of mast cells, and highest numbers of mast cells were seen in subepithelial zone followed by intermediate and deep zones. There was statistically significant relation in the number of mast cells in dentigerous cysts and dental follicle along subepithelial and intermediate zone with a value of <0.05. In our study, we also found increased mast cell count in inflamed cases of dental follicle and dentigerous cyst compared with noninflamed cases with a value of <0.01.
It is well known that mast cells play a role in the initiation of inflammation, and this inflammatory process may be associated with pericoronal follicle enlargement, a process that could result in cystic transformation of the follicle. Hence, regular radiographic follow-up is necessary especially for teeth with a maximum dental follicle width of 2-3 mm.
肥大细胞是大颗粒细胞，起源于骨髓中的多能CD 34+前体细胞，通常分布于整个结缔组织。在免疫或非免疫刺激激活后，肥大细胞释放分泌颗粒，用甲苯胺蓝染色呈现特征性的异染性外观。肥大细胞脱颗粒时释放的多种介质在牙源性囊肿的发病机制中起重要作用。
牙源性囊肿，如含牙囊肿，是由于未萌出牙的牙冠与缩余釉上皮之间积液形成的。围绕发育中牙齿的牙囊也可发生囊性转化。肥大细胞的活性可能导致囊肿扩大和骨吸收，突出了它们在囊性病变中的潜在作用。
研究牙囊和含牙囊肿中肥大细胞的存在情况。对上述研究对象中的肥大细胞进行定量。研究肥大细胞在研究组不同区域的分布模式。
这是一项于2012年至2015年在班加罗尔维迪希牙科学院和研究中心口腔病理学与微生物学系进行的组织病理学研究。
我们的研究于2012年至2015年在维迪希牙科学院和研究中心口腔病理学与微生物学系进行。对30例牙囊和含牙囊肿进行组织病理学分析，取4 - 5微米切片，用甲苯胺蓝染色。在三个不同区域（包括上皮下、中间和深部区域）对肥大细胞进行计数。结果列表并进行统计学分析。
克鲁斯卡尔 - 沃利斯卡方检验。
牙囊和含牙囊肿中均可见肥大细胞，上皮下区域肥大细胞数量最多，其次是中间区域和深部区域。在含牙囊肿和牙囊中，上皮下和中间区域肥大细胞数量存在统计学显著相关性，P值<0.05。在我们的研究中，还发现与非炎症性牙囊和含牙囊肿相比，炎症性牙囊和含牙囊肿中的肥大细胞计数增加，P值<0.01。
众所周知，肥大细胞在炎症起始中起作用，这种炎症过程可能与冠周牙囊扩大有关，而冠周牙囊扩大可能导致牙囊的囊性转化。因此，定期进行影像学随访是必要的，特别是对于牙囊最大宽度为2 - 3毫米的牙齿。
