Mansouri Sofiene
Department of Biomedical Technology, College of Applied Medical Sciences in Al-Kharj, Prince Sattam bin Abdulaziz University, Al-Kharj, Saudi Arabia.
Crit Rev Anal Chem. 2025 Apr 18:1-20. doi: 10.1080/10408347.2025.2491683.
In today's context, there is an increasing awareness among individuals regarding the importance of healthy and safe food consumption. Consequently, there is a growing demand for food products that are safeguarded against the detrimental effects of pathogens and harmful microbial metabolites. Actually, these organisms and their associated toxins pose a significant risk to food safety and are recognized as a critical threat to human health because of their capacity to induce foodborne infections and intoxications. Consequently, in order to address such challenges, it is imperative to enhance recognizing systems comprising bio/nanosensors for detections, which are trustworthy, quick, beneficial and economical. The advent of digital color imaging technology has led to the gradual establishment of lateral flow assays (LFAs) as one of the most significant sensors for point-of-care applications. Unlike colloidal gold nanoparticles (AuNPs), nanozymes offer enhanced color intensity through target-induced precise enrichment of nanozymes at the test line. Additionally, they amplify the color signal by facilitating the catalytic oxidation of colorless substrates into colored products. This dual functionality presents significant potential for the development of well-organized LFAs. In light of this, significant attempts are dedicated to the development of nanozyme-based LFAs. This review aims to outline recent advancements in the synthesis and design of nanozymes with varying compositions that exhibit distinct activities, as well as the structure and employment of nanozyme-based LFAs for the detection of pathogenic microorganisms and their associated toxins. Furthermore, the existing challenges and prospective development directions are outlined to assist readers in advancing the nanozyme-based LFAs performance.
在当今背景下，个人越来越意识到健康安全的食品消费的重要性。因此，对能抵御病原体和有害微生物代谢产物有害影响的食品的需求日益增长。实际上，这些生物体及其相关毒素对食品安全构成重大风险，并且由于它们能够引发食源性感染和中毒，被认为是对人类健康的严重威胁。因此，为应对此类挑战，必须加强包括用于检测的生物/纳米传感器的识别系统，这些系统应值得信赖、快速、有益且经济。数字彩色成像技术的出现导致侧向流动分析（LFA）逐渐成为即时检测应用中最重要的传感器之一。与胶体金纳米颗粒（AuNP）不同，纳米酶通过目标诱导的纳米酶在测试线上的精确富集提供增强的颜色强度。此外，它们通过促进无色底物催化氧化为有色产物来放大颜色信号。这种双重功能为开发组织良好的LFA提供了巨大潜力。有鉴于此，人们致力于开发基于纳米酶的LFA。本综述旨在概述具有不同活性的不同组成纳米酶的合成和设计的最新进展，以及基于纳米酶的LFA用于检测致病微生物及其相关毒素的结构和应用。此外，还概述了现有挑战和未来发展方向，以帮助读者提高基于纳米酶的LFA性能。
