使用逆转录定量聚合酶链反应（RT-qPCR）随后进行核苷酸测序和系统发育分析的黄热病病毒基因分型工具
Yellow Fever Virus Genotyping Tool Using RT-qPCR Followed by Nucleotide Sequencing and Phylogenetic Analysis.
作者信息
Rezende Izabela M, Sacchetto Lívia, Alves Pedro A, Labeaud A Desiree, Drumond Betânia P
机构信息
Department of Pediatrics, Division of Infectious Diseases, Stanford University School of Medicine, Stanford, CA, USA.
Laboratory of Viruses, Department of Microbiology, Federal University of Minas Gerais (UFMG), Belo Horizonte, Minas Gerais, Brazil.
出版信息
Methods Mol Biol. 2025;2913:153-166. doi: 10.1007/978-1-0716-4458-4_13.
Yellow fever is an infectious disease preventable by vaccination. The vaccine is composed of a live attenuated virus that, in some cases, may lead to adverse events, as neurological or viscerotropic disease. The differentiation between serious adverse vaccine events and natural infection by yellow fever virus is essential, especially in yellow fever endemic areas. Here we describe a genotyping tool to distinguish the vaccine from wild-type viruses. Experimental assays confirmed the discrimination between South American I and vaccine viruses, but in silico analyses also show its potential to be used for the differentiation of all yellow fever virus genotypes.
黄热病是一种可通过接种疫苗预防的传染病。该疫苗由减毒活病毒组成，在某些情况下，可能会导致不良事件，如神经型或嗜内脏型疾病。区分严重的疫苗不良事件和黄热病病毒的自然感染至关重要，尤其是在黄热病流行地区。在此，我们描述了一种基因分型工具，用于区分疫苗病毒和野生型病毒。实验分析证实了该工具可区分南美I型病毒和疫苗病毒，但计算机模拟分析也表明其有潜力用于区分所有黄热病病毒基因型。
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