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巴西用于黄热病病毒疫情监测的谱系特异性实时逆转录聚合酶链反应

Lineage-Specific Real-Time RT-PCR for Yellow Fever Virus Outbreak Surveillance, Brazil.

作者信息

Fischer Carlo, Torres Maria C, Patel Pranav, Moreira-Soto Andres, Gould Ernest A, Charrel Rémi N, de Lamballerie Xavier, Nogueira Rita Maria Ribeiro, Sequeira Patricia C, Rodrigues Cintia D S, Kümmerer Beate M, Drosten Christian, Landt Olfert, Bispo de Filippis Ana Maria, Drexler Jan Felix

出版信息

Emerg Infect Dis. 2017 Nov;23(11):1867-71. doi: 10.3201/eid2311.171131. Epub 2017 Nov 17.

DOI:10.3201/eid2311.171131
PMID:28949285
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5652426/
Abstract

The current yellow fever outbreak in Brazil prompted widespread yellow fever virus (YFV) vaccination campaigns, imposing a responsibility to distinguish between vaccine- and wild-type YFV-associated disease. We developed novel multiplex real-time reverse transcription PCRs that differentiate between vaccine and American wild-type YFV. We validated these highly specific and sensitive assays in an outbreak setting.

摘要

巴西当前爆发的黄热病促使开展了广泛的黄热病病毒（YFV）疫苗接种运动，这就带来了区分疫苗相关和野生型YFV所致疾病的责任。我们开发了新型多重实时逆转录PCR方法，用于区分疫苗株和美洲野生型YFV。我们在疫情环境中对这些高度特异且灵敏的检测方法进行了验证。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/219a/5652426/2d31fb9c229f/17-1131-F2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/219a/5652426/03d887931eca/17-1131-F1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/219a/5652426/2d31fb9c229f/17-1131-F2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/219a/5652426/03d887931eca/17-1131-F1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/219a/5652426/2d31fb9c229f/17-1131-F2.jpg

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Cells. 2025 Feb 24;14(5):334. doi: 10.3390/cells14050334.
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