秀丽隐杆线虫中的趋化性：探究气味剂对生理学的影响。

Chemotaxis in C. elegans: Interrogating the Effects of Odorants on Physiology.

作者信息

De-Souza Evandro A

机构信息

Department of Biochemistry and Tissue Biology, Institute of Biology, Universidade Estadual de Campinas (UNICAMP), Campinas, SP, Brazil.

Departamento de Bioquímica, Instituto de Química, Universidade de São Paulo, São Paulo, Brazil.

出版信息

Methods Mol Biol. 2025;2915:131-138. doi: 10.1007/978-1-0716-4466-9_7.

DOI:10.1007/978-1-0716-4466-9_7

PMID:40249487

Abstract

This chapter covers methodologies and materials commonly used for investigating chemotaxis behavior by odorants in Caenorhabditis elegans. Emphasis is placed on the role of odorants and their influence on the animal's physiology. Detailed assays are presented describing how to study the effects of odorants on worms' physiology and alterations in gene expression, particularly related to stress responses.

摘要

本章涵盖了常用于研究秀丽隐杆线虫中气味剂趋化行为的方法和材料。重点在于气味剂的作用及其对动物生理的影响。文中详细介绍了相关实验分析方法，描述了如何研究气味剂对蠕虫生理的影响以及基因表达的变化，特别是与应激反应相关的变化。