Andrews P M, Bates S B
Am J Kidney Dis. 1985 Jul;6(1):53-8. doi: 10.1016/s0272-6386(85)80038-x.
An in situ flushing solution was evaluated with regard to the following: (1) its ability to protect the kidney during 60, 90, and 120 minutes of normothermic ischemia; (2) the effects of using an intracellular versus extracellular electrolyte composition in the flushing solution; and (3) the ability of the flushing solution to complement in situ hypothermia as a protective measure during long-term ischemia. Rat kidneys were briefly flushed in situ with an isotonic phosphate buffered solution (pH 7.2) containing 50 milliosmole of sucrose. The left renal pedicle was then immediately clamped to render the kidney ischemic and to hold the flushing solution in the kidney. Following removal of the pedicle clamp, a contralateral nephrectomy of the right kidney was performed and daily serum creatinine levels determined to evaluate postischemic renal function. The results indicate the following: (1) the flushing procedure is very effective in preventing postischemic acute renal failure following 60 minutes of normothermic ischemia, but is considerably less effective for ischemic times of 90 minutes or more; (2) an intracellular electrolyte composition in the flushing solution does not improve the protective effects of this solution; and (3) the flushing procedure can significantly improve on the protection otherwise provided by in situ hypothermia.
(1) 其在正常体温下60、90和120分钟缺血期间保护肾脏的能力；(2) 在冲洗液中使用细胞内与细胞外电解质成分的效果；(3) 在长期缺血期间，冲洗液作为一种保护措施补充原位低温的能力。用含有50毫渗量蔗糖的等渗磷酸盐缓冲溶液（pH 7.2）对大鼠肾脏进行原位短暂冲洗。然后立即夹住左肾蒂使肾脏缺血，并将冲洗液留在肾脏中。在松开肾蒂夹后，对右肾进行对侧肾切除术，并测定每日血清肌酐水平以评估缺血后肾功能。结果表明：(1) 冲洗程序在预防正常体温下60分钟缺血后的缺血后急性肾衰竭方面非常有效，但对90分钟或更长时间的缺血效果要差得多；(2) 冲洗液中的细胞内电解质成分并不能改善该溶液的保护作用；(3) 冲洗程序可以显著改善原位低温提供的保护效果。
