Aung Hsu Po Po, Issarankura Na Ayudhaya Tawewan, Chaichoun Kridsada, Taowan Jarupha, Saechin Aeknarin, Buamas Supakit, Buddhirongawatr Ruangrat
Faculty of Graduate Studies, Mahidol University, Salaya, Phutthamonthon, Nakhon Pathom, Thailand.
Faculty of Veterinary Science, Mahidol University, Salaya, Phutthamonthon, Nakhon Pathom, Thailand.
Vet Q. 2025 Dec;45(1):1-9. doi: 10.1080/01652176.2025.2488025. Epub 2025 Apr 21.
This study aimed to classify the Feline leukaemia virus (FeLV) infection outcomes in domestic cats in Thailand and determine the accuracy of conjunctival swabs for FeLV proviral DNA detection by comparing results to PCR testing of blood samples. Whole blood and conjunctival swabs were collected from 126 cats with and without clinical signs. Blood specimens were evaluated for p27 FeLV antigen using the SNAP Feline Immunodeficiency Virus (FIV)/FeLV Combo Test, IDEXX Laboratories. The 3'-LTR region of the proviral FeLV was amplified from both blood and conjunctival samples. The prevalence rates of progressive and regressive FeLV infections in this study were 14.3% (95% CI: 8.69-21.63) and 36.5% (95% CI: 28.12-45.55), respectively. Cats older than 12 months of age had a higher probability of being regressively infected than cats younger than 1 year (-value = 0.039, OR =0.294, 95% CI: 0.092-0.942). Conjunctival swabs used for detecting FeLV proviral DNA demonstrated a sensitivity of 95.3% (95% CI: 86.91-99.02) and a specificity of 100% (95% CI: 94.22-100.00) compared to conventional blood samples. The observed kappa value of 0.956 indicates that conjunctival swabs are reliable and can be used as an alternative to blood venipuncture.
本研究旨在对泰国家猫的猫白血病病毒（FeLV）感染结果进行分类，并通过将结膜拭子检测结果与血液样本的聚合酶链反应（PCR）检测结果进行比较，来确定结膜拭子检测FeLV前病毒DNA的准确性。从126只有或没有临床症状的猫身上采集全血和结膜拭子。使用IDEXX实验室的SNAP猫免疫缺陷病毒（FIV）/FeLV联合检测法对血液样本进行p27 FeLV抗原评估。从血液和结膜样本中扩增前病毒FeLV的3'-长末端重复序列（LTR）区域。本研究中进行性和退行性FeLV感染的患病率分别为14.3%（95%置信区间：8.69 - 21.63）和36.5%（95%置信区间：28.12 - 45.55）。12个月龄以上的猫比1岁以下的猫发生退行性感染的可能性更高（P值 = 0.039，优势比 = 0.294，95%置信区间：0.092 - 0.942）。与传统血液样本相比，用于检测FeLV前病毒DNA的结膜拭子敏感性为95.3%（95%置信区间：86.91 - 99.02），特异性为100%（95%置信区间：94.22 - 100.00）。观察到的kappa值为0.956，表明结膜拭子是可靠的，可作为静脉穿刺采血的替代方法。
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