Mummoorthy Kunambiga, Yasmin Abd Rahaman, Arshad Siti Suri, Omar Abdul Rahman, Nur-Fazila Saulol Hamid, Anand Prem, Hoong Liew Wuan, Kumar Kiven
Gasing Veterinary Hospital, 53 and 55, Jalan 5/58, Gasing Indah, 46000 Petaling Jaya, Selangor, Malaysia.
Department of Veterinary Laboratory Diagnosis, Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia.
Vet World. 2021 Feb;14(2):405-409. doi: 10.14202/vetworld.2021.405-409. Epub 2021 Feb 13.
Feline leukemia virus (FeLV) is classified as . FeLV occurs worldwide, including Malaysia. Thus far, only one decade-old study on molecular characterization of Malaysian FeLV isolates exists, which resulted in a scarcity of updated information of current FeLV isolates circulating in Malaysia. This study was conducted to determine the status of FeLV in clinically ill cats and to study the molecular characterization and phylogenetic relatedness of the current isolates.
Convenience sampling was performed in 20 cats from the Gasing Veterinary Hospital in Selangor. Plasma and saliva samples were collected from 15 clinically ill cats and 5 healthy cats subjected to one-step reverse transcription-polymerase chain reaction with primers targeting a highly conserved gene of U3-LTR-gag.
Two clinically ill cats' plasma and saliva samples tested positive for FeLV RNA. Partial nucleotide sequencing and phylogenetic analysis revealed that the current isolates were 94-99% homologous to the previous Malaysian and Japanese FeLV isolates.
Current FeLV isolates from this study displayed higher similarity with the previous Malaysian isolates, signifying that a similar FeLV strain circulated among the cat population in Selangor.
猫白血病病毒（FeLV）被归类为……。FeLV在全球范围内存在，包括马来西亚。迄今为止，仅有一项关于马来西亚FeLV分离株分子特征的十年前的研究，这导致马来西亚目前流行的FeLV分离株的最新信息匮乏。本研究旨在确定临床患病猫中FeLV的状况，并研究当前分离株的分子特征和系统发育相关性。
在雪兰莪加星兽医医院对20只猫进行便利抽样。从15只临床患病猫和5只健康猫采集血浆和唾液样本，使用针对U3-LTR-gag高度保守基因的引物进行一步逆转录聚合酶链反应。
两只临床患病猫的血浆和唾液样本检测出FeLV RNA呈阳性。部分核苷酸测序和系统发育分析表明，当前分离株与先前的马来西亚和日本FeLV分离株有94% - 99%的同源性。
本研究中当前的FeLV分离株与先前的马来西亚分离株显示出更高的相似性，这表明雪兰莪猫群中存在相似的FeLV毒株。
