Li Qi-You, Tan Xiao-Ling, Xu Hai-Wei, Zeng Yu-Xiao, Huang Xiao-Yong
Southwest Hospital/Southwest Eye Hospital, Third Military Medical University (Army Medical University), Chongqing 400038, China.
Key Lab of Visual Damage and Regeneration & Restoration of Chongqing, Chongqing 400038, China.
Int J Ophthalmol. 2025 Apr 18;18(4):582-589. doi: 10.18240/ijo.2025.04.03. eCollection 2025.
To explore the impact of insulin-like growth factor-1 receptor α (IGF-1Rα) on the differentiation fate of optic-cup-derived retinal stem cells (OC-RSCs) into retinal ganglion cells (RGCs) .
OC-RSCs were isolated from optic cups of rats on embryonic day 12.5, and high-purity OC-RSCs were obtained by conditioned culture and passage. Differentiation of OC-RSCs into RGCs under different serum concentrations was examined using flow cytometry, and the serum concentration with high interference with differentiation ratio was selected. Furthermore, the effect of blocking IGF-1Rα on the differentiation of OC-RSCs into RGCs was analyzed through immunocytochemistry and Western blotting.
Immunohistochemical analysis revealed IGF-1Rα was highly expressed in rat embryos at day 12.5. OC-RSCs were isolated and purified, and high-purity OC-RSCs were obtained. When 2.5% serum was administered, the ratio of differentiated RGCs (Thy-1.1 positive) decreased significantly, and the results of immunoblotting also confirmed the blockade of IGF-1Rα reduced Thy-1.1 protein expression.
IGF-1Rα blocking can reduce the differentiation of OC-RSCs into RGCs.
探讨胰岛素样生长因子-1受体α(IGF-1Rα)对视杯来源的视网膜干细胞(OC-RSCs)向视网膜神经节细胞(RGCs)分化命运的影响。
从胚胎第12.5天大鼠的视杯中分离OC-RSCs,通过条件培养和传代获得高纯度的OC-RSCs。采用流式细胞术检测不同血清浓度下OC-RSCs向RGCs的分化情况,选取对分化率干扰较大的血清浓度。此外,通过免疫细胞化学和蛋白质免疫印迹法分析阻断IGF-1Rα对OC-RSCs向RGCs分化的影响。
免疫组织化学分析显示,IGF-1Rα在胚胎第12.5天的大鼠胚胎中高表达。分离并纯化了OC-RSCs,获得了高纯度的OC-RSCs。当给予2.5%血清时,分化的RGCs(Thy-1.1阳性)比例显著降低,免疫印迹结果也证实阻断IGF-1Rα可降低Thy-1.1蛋白表达。
阻断IGF-1Rα可减少OC-RSCs向RGCs的分化。