胆汁酸。第七十二部分。胆汁酸辅酶A衍生物的高效液相色谱分析。

Bile acids. LXXII. High-performance liquid chromatographic analysis of bile acid coenzyme A derivatives.

作者信息

Abbott D A, Schlarman D E, Patrick P H, Tal D M, Elliott W H

出版信息

Anal Biochem. 1985 May 1;146(2):437-41. doi: 10.1016/0003-2697(85)90566-4.

Abstract

The mobilities of coenzyme A and coenzyme A derivatives of cholate, chenodeoxycholate, deoxycholate, lithocholate, and their 5 alpha analogs were studied in reversed-phase high-performance liquid chromatography. With a C18 Radial-PAK A cartridge (10-micron particles) and a solvent mixture of 2-propanol/10 mM phosphate buffer (pH 7.0, 140:360), separation of the chenodeoxycholyl and deoxycholyl coenzyme A derivatives was not observed. An increase in ionic strength of the buffer to 50 mM afforded separation, which was markedly augmented with a C18 Radial-PAK A cartridge with 5-micron particles. Lowering the pH of the buffer to 5.5 did not materially change the separations regardless of the ionic strength. Quantitation was carried out to a lower level of 8.5 X 10(-12) mol.

摘要

在反相高效液相色谱法中研究了辅酶A以及胆酸盐、鹅去氧胆酸盐、脱氧胆酸盐、石胆酸盐及其5α类似物的辅酶A衍生物的迁移率。使用C18径向Pak A柱（10微米颗粒）和2-丙醇/10 mM磷酸盐缓冲液（pH 7.0，140:360）的溶剂混合物时，未观察到鹅去氧胆酰辅酶A衍生物和脱氧胆酰辅酶A衍生物的分离。将缓冲液的离子强度增加到50 mM可实现分离，使用5微米颗粒的C18径向Pak A柱时分离效果明显增强。无论离子强度如何，将缓冲液的pH降低到5.5并不会实质性地改变分离效果。定量下限为8.5×10⁻¹² mol。