Shimada K, Hasegawa M, Goto J, Nambara T
J Chromatogr. 1978 May 21;152(2):431-6. doi: 10.1016/s0021-9673(00)85079-7.
A method is described for the simultaneous determination of major bile acids by high-performance liquid chromatography without prior hydrolysis. A mixture of bile acids is divided into the free, glyco- and tauro-conjugate groups by thin-layer chromatography. Separation of each group into cholate, ursodeoxycholate, chenodeoxycholate, deoxycholate and lithocholate is attained in two stages on a muBondapak C18 column; first, 0.3% ammonium carbonate-acetonitrile (9:4) is used as a mobile phase for the separation of the last three compounds. Subsequently cholate and ursodeoxycholate are resolved by chromatography in 0.3% ammonium carbonate-acetonitrile (11:4).
描述了一种无需预先水解即可通过高效液相色谱法同时测定主要胆汁酸的方法。胆汁酸混合物通过薄层色谱法分为游离型、甘氨共轭型和牛磺共轭型。在μBondapak C18柱上分两个阶段将每组分离为胆酸盐、熊去氧胆酸盐、鹅去氧胆酸盐、脱氧胆酸盐和石胆酸盐；首先，使用0.3%碳酸铵-乙腈（9:4）作为流动相分离后三种化合物。随后，通过在0.3%碳酸铵-乙腈（11:4）中进行色谱分离来分离胆酸盐和熊去氧胆酸盐。
