内皮细胞c-Myc与阿霉素诱导的代谢改变：一项多器官光学成像研究
Endothelial c-Myc and Doxorubicin-Induced Metabolic Alterations: A Multi-Organ Optical Imaging Study.
作者信息
Nategh Parisa, Neghabi Mehrnoosh, Ceyhan Busenur, Machi Jacqueline F, Rahbar Homan A, Rodriguez Maya S, Santana Aline G, Rodrigues Claudia O, Ranji Mahsa
机构信息
Biophotonics Lab, Department of Electrical Engineering and Computer Science, Florida Atlantic University, Boca Raton, Florida, USA.
Department of Biomedical Science, Schmidt College of Medicine, Florida Atlantic University, Boca Raton, Florida, USA.
出版信息
J Biophotonics. 2025 Apr 21:e70037. doi: 10.1002/jbio.70037.
SIGNIFICANCE
Endothelial c-Myc plays a critical role in redox homeostasis, with its deletion potentially exacerbating oxidative stress, particularly, during chemotherapy.
AIM
To assess the metabolic impact of endothelial c-Myc knockout (KO) and Doxorubicin (DOXO) treatment on kidneys, hearts, and livers using 3D optical cryo-imaging.
APPROACH
Redox ratios (NADH/FAD) were analyzed in control (CT) and KO mice treated with DOXO or saline to evaluate mitochondrial function and oxidative states.
RESULTS
KO tissues showed significant reductions in redox ratios, indicating an oxidized state, with kidneys exhibiting up to a 51.42% decrease. DOXO treatment further exacerbated oxidative stress in KO tissues, while CT groups demonstrated protective effects.
CONCLUSIONS
Endothelial c-Myc is crucial for redox balance and protection against chemotherapy-induced oxidative damage, offering insights for targeted therapeutic strategies.
意义
内皮细胞c-Myc在氧化还原稳态中起关键作用，其缺失可能会加剧氧化应激，尤其是在化疗期间。
目的
使用3D光学冷冻成像评估内皮细胞c-Myc基因敲除（KO）和多柔比星（DOXO）治疗对肾脏、心脏和肝脏的代谢影响。
方法
分析用DOXO或生理盐水处理的对照（CT）小鼠和KO小鼠的氧化还原比（NADH/FAD），以评估线粒体功能和氧化状态。
结果
KO组织的氧化还原比显著降低，表明处于氧化状态，肾脏的氧化还原比降低高达51.42%。DOXO治疗进一步加剧了KO组织中的氧化应激，而CT组则表现出保护作用。
结论
内皮细胞c-Myc对氧化还原平衡和预防化疗诱导的氧化损伤至关重要，为靶向治疗策略提供了思路。
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