Saghafi Mohammad, Tripathy Suryasnata, Moazzenzade Taghi, Huskens Jurriaan, Lemay Serge G
Department of Molecules and Materials, Faculty of Science and Technology, University of Twente, 7500 AE Enschede, The Netherlands.
Nano Lett. 2025 Jun 25;25(25):9891-9898. doi: 10.1021/acs.nanolett.4c05324. Epub 2025 Apr 22.
CMOS-based nanocapacitor arrays are an emerging technology that permits spatially resolved, high-frequency impedance measurements at the nanoscale. Their capability to detect micro- and nanoscale entities has already been established through nonspecific interactions with the targets. Here, we demonstrate their application in specific macromolecular capture and detection using single-stranded DNA (ssDNA) as a model analyte. While individual ssDNA strands fall below the detection threshold, we employ a strand displacement assay that links DNA hybridization to target ssDNA induced displacement of reporter nanoparticles. This displacement reaction results in distinct electrical signatures with complex spatiotemporal patterns, details that remain unresolved in conventional macroscale impedance spectroscopy techniques due to their limited resolution and signal averaging that obscures localized interactions. The proposed system's massively parallel architecture and the ability to detect complex dynamics of individual nanoparticle-nanoelectrode interactions make it a promising candidate for scalable, portable, and cost-effective biosensing applications in clinical diagnostics and beyond.
基于互补金属氧化物半导体（CMOS）的纳米电容器阵列是一种新兴技术，它能够在纳米尺度上进行空间分辨的高频阻抗测量。通过与目标的非特异性相互作用，其检测微米和纳米级实体的能力已经得到证实。在这里，我们展示了它们在使用单链DNA（ssDNA）作为模型分析物的特定大分子捕获和检测中的应用。虽然单个ssDNA链低于检测阈值，但我们采用了一种链置换分析方法，该方法将DNA杂交与报告纳米颗粒的靶标ssDNA诱导置换联系起来。这种置换反应产生具有复杂时空模式的独特电信号，由于传统宏观阻抗谱技术分辨率有限且信号平均会掩盖局部相互作用，这些细节在传统宏观阻抗谱技术中仍未得到解决。所提出系统的大规模并行架构以及检测单个纳米颗粒与纳米电极相互作用复杂动态的能力，使其成为临床诊断及其他领域中可扩展、便携式且经济高效的生物传感应用的有前途候选者。
