School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai 200030, People's Republic of China.
Department of Instrument Science and Engineering, School of Electronic Information and Electrical Engineering, Shanghai Jiao Tong University, Shanghai 200030, People's Republic of China.
Proc Natl Acad Sci U S A. 2022 Mar 8;119(10):e2120379119. doi: 10.1073/pnas.2120379119. Epub 2022 Mar 1.
SignificanceThe detection of low-abundance molecular biomarkers is key to the liquid-biopsy-based disease diagnosis. Existing methods are limited by the affinity and specificity of recognition probes and the mass transportation of analyte molecules onto the sensor surfaces, resulting in insufficient sensitivity and long assay time. This work establishes a rapid and ultrasensitive approach by actively tuning binding kinetics and accelerating the mass transportation via nanoparticle micromanipulations. This is significant because it permits extremely sensitive measurements within clinically acceptable assay time. It is incubation-free, washing-free, and compatible with low- and high-affinity probes.
低丰度分子生物标志物的检测是基于液体活检的疾病诊断的关键。现有的方法受到识别探针的亲和力和特异性以及分析物分子在传感器表面上的质量传输的限制，导致灵敏度不足和分析时间长。本工作通过主动调整结合动力学并通过纳米颗粒微操作加速质量传输，建立了一种快速和超灵敏的方法。这是重要的，因为它允许在临床可接受的分析时间内进行极其灵敏的测量。它是无孵育、无洗涤的，并且与低亲和性和高亲和性探针兼容。
