Thaler M M, Wennberg R P
Pediatr Res. 1977 Mar;11(3 Pt 1):167-71. doi: 10.1203/00006450-197703000-00004.
The effects of an emulsified intravenous fat preparation (Intralipid) on bilirubin transport were analyzed by cholestyramine extraction, spectrophotometric analysis, Sephadex gel filtration, peroxidase assay, bilirubin uptake by red blood cells, and by toxicity in tissue culture (L-929) cells. Intralipid is capable of binding bilirubin, but does not compete effectively with bilirubin bound to high affinity sites on albumin. The emulsified fat appears to have a higher affinity for bilirubin than for cell membranes. Red blood cells become coated with Intralipid, resulting in an increased association of bilirubin with the cell surface, but a decrease in pigment actually incorporated into the red cell membrane. In tissue culture experiments, Intralipid protected the cells from bilirubin toxicity. It is concluded that Intralipid may enhance the carrying capacity of serum for bilirubin, and thus exert a protective effect on tissues.
通过消胆胺萃取、分光光度分析、葡聚糖凝胶过滤、过氧化物酶测定、红细胞对胆红素的摄取以及组织培养（L-929）细胞毒性分析，研究了乳化静脉脂肪制剂（英脱利匹特）对胆红素转运的影响。英脱利匹特能够结合胆红素，但不能有效地与白蛋白上高亲和力位点结合的胆红素竞争。乳化脂肪对胆红素的亲和力似乎高于对细胞膜的亲和力。红细胞被英脱利匹特包裹，导致胆红素与细胞表面的结合增加，但实际掺入红细胞膜的色素减少。在组织培养实验中，英脱利匹特保护细胞免受胆红素毒性。得出的结论是，英脱利匹特可能增强血清对胆红素的携带能力，从而对组织发挥保护作用。
