Shennan A T, Cherian A G, Angel A, Bryan M H
J Pediatr. 1976 Feb;88(2):285-8. doi: 10.1016/s0022-3476(76)81001-3.
After noting rises in measured serum billirubin in premature infants undergoing parenteral nutrition, a series of experiments was designed to ascertain the effect of the presence of Intralipid on the actual laboratory analysis. It has been shown that the most commonly used spectrophotometric analysis of bilirubin and the estimation of direct bilirubin by micromethods are affected by the presence of Intralipid in the specimens. Centrifugation of the blood samples at 15,000 rpm for 10 minutes and analysis of the aqueous layer overcomes, in both methods, the interference and error introduced by the presence of Intralipid chylomicrons in patient sera. Alternatively spurious bilirubin measurements due to hyperlipidemia may be avoided by using a bilirubinometer equipped with a filter which eliminates the effect of Intralipid interference.
在注意到接受肠外营养的早产儿血清胆红素测量值升高后，设计了一系列实验来确定脂肪乳剂的存在对实际实验室分析的影响。结果表明，最常用的胆红素分光光度分析方法以及微量法测定直接胆红素均受标本中脂肪乳剂的影响。两种方法中，将血样以15000转/分钟的速度离心10分钟并分析水层，可克服患者血清中脂肪乳剂乳糜微粒的存在所带来的干扰和误差。另外，使用配备可消除脂肪乳剂干扰影响的滤光器的胆红素测定仪，可避免因高脂血症导致的假性胆红素测量结果。
