耐抗菌药物的布鲁氏菌属在不同畜牧场的生牛奶和动物粪便中普遍存在。

Antimicrobial resistant Brucella spp. prevail in raw milk and animal feces of different livestock farms.

作者信息

Zeb Shaista, Yasmin Humaira, Malik Imran Riaz, Farah Mohammad Abul, Arya Vivak M, Hassan Muhammad Nadeem

机构信息

Department of Biosciences, COMSATS University Islamabad (CUI), Park Road, Islamabad, Pakistan.

Department of Biotechnology, University of Sarghodha, Sargodha, Pakistan.

出版信息

BMC Microbiol. 2025 Apr 22;25(1):231. doi: 10.1186/s12866-025-03930-8.

DOI:10.1186/s12866-025-03930-8

PMID:40263991

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12013057/

Abstract

BACKGROUND

Brucella spp. is a zoonotic pathogen that affects both livestock and humans, causing reproductive issues in animals and severe health complications in humans, including undulant fever, hepatomegaly, and arthritis. Contaminated raw milk and feces serve as potential transmission sources, despite its public health significance, limited studies have assessed the prevalence of Brucella spp. in raw milk and feces, particularly in endemic regions like Pakistan.

OBJECTIVE

This study aimed to determine the prevalence of Brucella spp. in raw milk and feces of livestock in Punjab and Islamabad Capital Territory, Pakistan, and evaluate the antibiotic resistance profiles of the isolated strains.

METHODS

The raw milk and fecal samples were collected randomly from the different livestock farms of Punjab, Pakistan. The areas were selected based on the different sociodemographic attributes like climate, land usage, number of animals, husbandry practices and operational convenience, which may influence the spatial and temporal distribution of livestock diseases. Brucella spp. was isolated using Brucella agar, a highly specific medium, and confirmed at the molecular level through IS711 gene analysis. Antibiotic susceptibility testing was performed to determine the multiple antibiotic resistance (MAR) index and identify multidrug-resistant (MDR) strains.

RESULTS

A total of 100 samples, including 60 raw milk samples (cows = 25, buffalo = 25, goats = 10) and 40 fecal samples (cows = 16, buffalo = 15, goats = 9), were collected from various livestock farms. The incidence of Brucella spp. was found higher (7.5%) in raw milk than in the animal´s feca (15%). All the strains showed multiple antibiotic resistance (MAR) index (0.3-0.5) which was higher than the critical value (> 0.2). Additionally, 75% of the strains were classified as multidrug-resistant (MDR), highlighting their resistance to multiple antibiotics.

CONCLUSION

These findings indicate the antimicrobial resistance and prevalence of Brucella spp. in the dairy industry which could be a serious threat to public health.

CLINICAL TRIAL NUMBER

Not applicable.

摘要

背景

布鲁氏菌属是一种人畜共患病原体，可感染家畜和人类，导致动物繁殖问题以及人类出现严重健康并发症，包括波状热、肝肿大和关节炎。尽管生牛奶和粪便具有公共卫生意义，但受污染的生牛奶和粪便仍是潜在的传播源，针对布鲁氏菌属在生牛奶和粪便中的流行情况，尤其是在巴基斯坦等流行地区，相关研究较少。

目的

本研究旨在确定巴基斯坦旁遮普省和伊斯兰堡首都地区家畜生牛奶和粪便中布鲁氏菌属的流行情况，并评估分离菌株的抗生素耐药谱。

方法

从巴基斯坦旁遮普省的不同家畜养殖场随机采集生牛奶和粪便样本。根据气候、土地用途、动物数量、饲养方式和操作便利性等不同社会人口学属性选择这些地区，这些属性可能会影响家畜疾病的时空分布。使用布鲁氏菌琼脂（一种高度特异性培养基）分离布鲁氏菌属，并通过IS711基因分析在分子水平上进行确认。进行抗生素敏感性测试以确定多重抗生素耐药（MAR）指数并鉴定多重耐药（MDR）菌株。

结果

共从各个家畜养殖场采集了100份样本，包括60份生牛奶样本（奶牛 = 25份，水牛 = 25份，山羊 = 10份）和40份粪便样本（奶牛 = 16份，水牛 = 15份，山羊 = 9份）。发现生牛奶中布鲁氏菌属的发生率（7.5%）高于动物粪便中的发生率（15%）。所有菌株均显示多重抗生素耐药（MAR）指数（0.3 - 0.5），高于临界值（> 0.2）。此外，75%的菌株被归类为多重耐药（MDR），突出了它们对多种抗生素的耐药性。