Dadar Maryam, Alamian Saeed
Agricultural Research, Education and Extension Organization (AREEO), Razi Vaccine and Serum Research Institute (RVSRI), Karaj, Iran.
Curr Microbiol. 2025 Jan 8;82(2):74. doi: 10.1007/s00284-024-03940-1.
Brucellosis, a zoonotic disease caused by Brucella spp. globally, is of great significance not only to livestock but also to public health. The most significant of the twelve species is Brucella melitensis. This article is devoted to the endemic region of Iran and aims to uncover the molecular epidemiology of B. melitensis. Biotyping, AMOS-PCR, Bruce-ladder PCR, and the in silico method of MLVA were employed to test 40 B. melitensis isolates from humans, cows, sheep, goats, camels, and horses which are found in thirteen Iranian provinces throughout the years 2015 to 2022. The data from the MLVA-8 analysis showed that there were seven genotypes that could be identified, and the most commonly identified genotype was genotype 63. The data from the MLVA-10 analysis showed that there were seven genotypes, with genotype 213 being the most prevalent in Iran. The data from the MLVA-11 analysis showed that there were eight genotypes, with genotype 111 being the most prevalent in Iran. The MLVA and SNP analysis results showed that the bacteria were grouped into two main groups, known as the Eastern Mediterranean and American groups. Moreover, the outcomes from these analyses have added considerably to our understanding of the genetic/historical relationships among the isolates. Our study indicates a high prevalence of B. melitensis biovar 1 in Iran, accounting for 82.5% of the isolates. The study provides insight into such matters as the complex epidemiology of B. melitensis in Iran, suggesting different ways of transmission and sources of infection. This research points out the vital significance of the continuation of the surveillance and curation of B. melitensis in the diverse species of animals and humans. The simplicity and efficiency of MLVA-based molecular epidemiology offer information on the geographic distribution and genetic diversity of B. melitensis and, therefore, help in the devising of targeted strategies for the prevention of disease in animals.
布鲁氏菌病是一种由布鲁氏菌属引起的人畜共患病，在全球范围内，不仅对家畜而且对公共卫生都具有重要意义。十二个物种中最重要的是羊布鲁氏菌。本文聚焦于伊朗的流行地区，旨在揭示羊布鲁氏菌的分子流行病学。采用生物分型、AMOS-PCR、布鲁氏菌阶梯PCR以及MLVA的计算机模拟方法，对2015年至2022年期间在伊朗十三个省份采集的来自人类、奶牛、绵羊、山羊、骆驼和马的40株羊布鲁氏菌分离株进行检测。MLVA-8分析的数据显示，可以识别出七种基因型，最常见的基因型是基因型63。MLVA-10分析的数据显示有七种基因型，基因型213在伊朗最为普遍。MLVA-11分析的数据显示有八种基因型，基因型111在伊朗最为普遍。MLVA和SNP分析结果表明，这些细菌被分为两个主要群体，即东地中海群体和美洲群体。此外，这些分析结果极大地增进了我们对分离株之间遗传/历史关系的理解。我们的研究表明，伊朗羊布鲁氏菌生物变种1的流行率很高，占分离株的82.5%。该研究深入了解了伊朗羊布鲁氏菌复杂的流行病学情况，提示了不同的传播方式和感染源。这项研究指出了持续监测和管理动物和人类不同物种中羊布鲁氏菌的至关重要性。基于MLVA的分子流行病学的简单性和有效性提供了关于羊布鲁氏菌地理分布和遗传多样性的信息，因此有助于制定针对性的动物疾病预防策略。
