Li Haiqin, Huang Haoyu, Lin Cui, Zhang Fanfan, Tan Jia, Li Na, Wu Chengcheng, Huang Jiangnan, Wei Qipeng, Wang Siyi, Zhu Yujun, Zeng Yanbing
Institute of Animal Husbandry and Veterinary Medicine, Jiangxi Academy of Agricultural Sciences, Nanchang, Jiangxi, 330200, China.
Guangdong Provincial Biotechnology Research Institute, Guangzhou, Guangdong, 510633, China.
BMC Vet Res. 2025 Apr 23;21(1):285. doi: 10.1186/s12917-025-04687-y.
Goose astrovirus genotype 2 (GoAstV-2) is a novel and highly infectious pathogen characterized by symptoms of gout, swelling, and hemorrhage of the kidneys in 5-15-day-old goslings. The virus has caused severe economic losses for the goose husbandry industry. The capsid protein of GoAstV-2 is involved in viral packaging and immunogenicity, and it has served as a target antigen for diagnostic methods.
In this study, the N-terminus of the GoAstV-2 Cap protein (rCap) was successfully expressed and used to immunize BALB/c mice. A new monoclonal antibody (mAb) named 7B2 against the GoAstV-2 rCap protein was prepared. Immunofluorescence assay and Western blot analysis showed that mAb 7B2 had a strong binding affinity to the GoAstV-2 rCap protein. The results of epitope mapping demonstrated that the minimum linear epitope recognized by mAb 7B2 was located in the amino acids NTAGPESIDT. Sequence homology analysis revealed that this epitope was highly conserved among the GoAstV-2 strains analyzed and that it showed 90% sequence similarity with turkey astrovirus 2 strains and low similarity (less than 50%) with other astroviruses.
These findings will be useful for the development of diagnostic kits for GoAstV-2 and will aid further research on the structure and function of the capsid protein.
鹅星状病毒2型（GoAstV-2）是一种新型高传染性病原体，其特征是5至15日龄雏鹅出现痛风、肾脏肿胀和出血症状。该病毒给养鹅业造成了严重的经济损失。GoAstV-2的衣壳蛋白参与病毒包装和免疫原性，已作为诊断方法的靶抗原。
在本研究中，成功表达了GoAstV-2 Cap蛋白（rCap）的N端，并用于免疫BALB/c小鼠。制备了一种针对GoAstV-2 rCap蛋白的新型单克隆抗体（mAb）7B2。免疫荧光分析和蛋白质印迹分析表明，mAb 7B2与GoAstV-2 rCap蛋白具有很强的结合亲和力。表位作图结果表明，mAb 7B2识别的最小线性表位位于氨基酸NTAGPESIDT中。序列同源性分析表明，该表位在所分析的GoAstV-2毒株中高度保守，与火鸡星状病毒2型毒株具有90%的序列相似性，与其他星状病毒的相似性较低（低于50%）。
这些发现将有助于开发GoAstV-2诊断试剂盒，并有助于进一步研究衣壳蛋白的结构和功能。
