College of Animal Science, Anhui Science and Technology University and Anhui Province Key Laboratory of Animal Nutritional Regulation and Health, Fengyang 233100, PR China.
Guangdong Laboratory Animals Monitoring Institute and Guangdong Provincial Key Laboratory of Laboratory Animals, Guangzhou 510663, PR China.
Int J Biol Macromol. 2023 Jun 1;239:124241. doi: 10.1016/j.ijbiomac.2023.124241. Epub 2023 Mar 29.
Swine acute diarrhea syndrome coronavirus (SADS-CoV), which causes severe diarrhea in newborn piglets, was first identified in Southern China in 2017. Since the Nucleocapsid (N) protein in SADS-CoV is highly conserved and plays a key role in virus replication, it is often used as a target protein in scientific research. In this study, the N protein of SADS-CoV was successfully expressed, and a new monoclonal antibody (mAb), 5G12, against the protein was generated successfully. The mAb 5G12 can be used to detect SADS-CoV strains by indirect immunofluorescence assay (IFA) and western blotting. The mAb 5G12 epitope was located to amino acids 11 EQAESRGRK 19 by evaluating the antibody for reactivity with a series of truncated N protein segments. The biological information analysis showed that the antigenic epitope had a high antigenic index and conservation. This study will help further understand the protein structure and function of SADS-CoV and in the establishment of specific SADS-CoV detection methods.
猪急性腹泻综合征冠状病毒（SADS-CoV）于 2017 年在中国南方首次被鉴定出来，可导致新生仔猪严重腹泻。由于 SADS-CoV 的核衣壳（N）蛋白高度保守，在病毒复制中发挥关键作用，因此常被用作科学研究的靶标蛋白。本研究成功表达了 SADS-CoV 的 N 蛋白，并成功生成了一种针对该蛋白的新型单克隆抗体（mAb）5G12。mAb 5G12 可通过间接免疫荧光法（IFA）和 Western blot 用于检测 SADS-CoV 株。通过评估该抗体与一系列截断的 N 蛋白片段的反应性，确定 mAb 5G12 的表位位于 11 EQAESRGRK 19 氨基酸处。生物信息学分析表明，该抗原表位具有较高的抗原指数和保守性。本研究将有助于进一步了解 SADS-CoV 的蛋白结构和功能，并建立针对 SADS-CoV 的特异性检测方法。
