Chankitisakul Vibuntita, Authaida Supakorn, Boonkum Wuttigrai, Tuntiyasawasdikul Sarunya
Department of Animal Science, Faculty of Agriculture, Khon Kaen University, Khon Kaen, Thailand.
Network Center for Animal Breeding and Omics Research, Khon Kaen University, Khon Kaen, Thailand.
Front Vet Sci. 2025 Apr 8;12:1582200. doi: 10.3389/fvets.2025.1582200. eCollection 2025.
The present study aimed to characterize the pharmacokinetics and stability of methoxyflavones derived from (KP) in Thai native roosters after oral administration of a KP ethanolic extract.
Twenty-seven male roosters were randomly divided into three groups and received KP extract at different doses of 100, 150, and 200 mg/kg of body weight. Plasma samples were prepared using acetonitrile, ethyl acetate, and a mixture of both to compare the optimal extraction efficiency. Plasma methoxyflavones concentrations were quantified using a validated HPLC method. Pharmacokinetic parameters were calculated using PKSolver. A seven-day stability study assessed methoxyflavones degradation in blood and plasma samples stored at -20°C.
The results showed that methoxyflavones were rapidly absorbed, reaching maximum plasma concentrations (C) ranging from 0.34 to 0.83 µg/mL within 1.17 to 1.83 hours, with a clear dose-dependent relationship. Elimination was slow, with half-lives ranging from 2.03 to 2.60 hours. The study also found that acetonitrile was the most effective solvent for extracting methoxyflavones from blood samples, yielding recovery rates of 73.95%, 81.49%, and 77.5% for 3,5,7,3',4'-pentamethoxyflavone (PMF), 5,7-dimethoxyflavone (DMF), and 5,7,4'-trimethoxyflavone (TMF), respectively. High stability was observed in blood and plasma over two days (96.6-100%), with significant degradation (84.3-92.6%) after seven days. This study's results provide valuable insights for optimizing KP extract use as a poultry feed additive by informing appropriate dosage, extraction, and storage procedures to preserve methoxyflavones integrity.
本研究旨在表征口服KP乙醇提取物后，泰国本地公鸡体内源自KP的甲氧基黄酮的药代动力学和稳定性。
将27只雄性公鸡随机分为三组，分别给予100、150和200mg/kg体重的不同剂量KP提取物。使用乙腈、乙酸乙酯以及两者的混合物制备血浆样品，以比较最佳提取效率。采用经过验证的高效液相色谱法对血浆中甲氧基黄酮浓度进行定量。使用PKSolver计算药代动力学参数。进行为期七天的稳定性研究，评估储存于-20°C的血液和血浆样品中甲氧基黄酮的降解情况。
结果表明，甲氧基黄酮吸收迅速，在1.17至1.83小时内达到0.34至0.83μg/mL的最大血浆浓度（Cmax），具有明显的剂量依赖性关系。消除缓慢，半衰期为2.03至2.60小时。该研究还发现，乙腈是从血液样品中提取甲氧基黄酮的最有效溶剂，对于3,5,7,3',4'-五甲氧基黄酮（PMF）、5,7-二甲氧基黄酮（DMF）和5,7,4'-三甲氧基黄酮（TMF），回收率分别为73.95%、81.49%和77.5%。在两天内血液和血浆中观察到高稳定性（96.6 - 100%），七天后出现显著降解（84.3 - 92.6%）。本研究结果为优化将KP提取物用作家禽饲料添加剂提供了有价值的见解，通过告知适当的剂量、提取和储存程序以保持甲氧基黄酮的完整性。
