Watts J W, Dawson J R, King J M
Ciba Found Symp. 1981;80:56-71. doi: 10.1002/9780470720639.ch5.
Plant protoplasts may be efficiently infected with viruses. Attachment to the plasmalemma is a critical step in infection; positively charged viruses readily adhere and infect but negatively charged viruses require the presence of a polycation (e.g. poly-L-ornithine) before infection can occur. The role of the polycation appears to be twofold: to modify the charge of the virus and to damage the surface of the protoplast. Virus probably enters the protoplast during repair of damaged regions of the plasmalemma. Pinocytosis does not appear to play a significant part in inoculation.
植物原生质体可被病毒有效感染。附着于质膜是感染过程中的关键步骤；带正电荷的病毒易于附着并感染，但带负电荷的病毒在感染发生前需要有聚阳离子（如聚-L-鸟氨酸）存在。聚阳离子的作用似乎有两方面：改变病毒的电荷以及损伤原生质体的表面。病毒可能在质膜受损区域修复过程中进入原生质体。胞饮作用似乎在接种过程中不起重要作用。
