Islam Mohammad Shafiqul, Ahmed Md Robel, Noman Muhammad, Zhang Zhen, Wang Jing, Lu Ziqi, Cai Yingying, Ahmed Temoor, Li Bin, Wang Yanli, Golam Sarwar Abul Khayer Mohammad, Wang Jiaoyu
State Key Laboratory for Quality and Safety of Agro-Products, Key Laboratory of Agricultural Microbiome of MARA and Zhejiang Province, Key Laboratory of Biotechnology in Plant Protection of MARA and Zhejiang Province, Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China.
State Key Laboratory of Rice Biology and Breeding, Ministry of Agriculture and Rural Affairs Key Laboratory of Molecular Biology of Crop Pathogens and Insect Pests, Zhejiang Key Laboratory of Biology and Ecological Regulation of Crop Pathogens and Insects, Institute of Biotechnology, Zhejiang University, Hangzhou 310058, China.
Plants (Basel). 2025 Mar 20;14(6):977. doi: 10.3390/plants14060977.
RNA interference (RNAi) has emerged as a potent mechanism for combating pathogenic fungi and oomycetes over the past decades. It offers a promising gene-silencing approach by targeting crucial genes involved in diseases caused by economically and scientifically significant fungal pathogens, such as and species. Simultaneously, nano-agro-products have gained attention as alternatives to traditional fungicides in plant protection strategies. However, the instability of naked RNA molecules outside the cellular environment presents a challenge, as they degrade rapidly, limiting their efficacy for prolonged disease control. Concerns regarding the toxicity of protective nanoparticles to non-target organisms have also arisen. Integrating RNAi with nano-agro-products, particularly nanocarriers, to form RNA-nano complexes has demonstrated significant potential, providing enhanced RNA stability, reduced toxicity, and extended disease control. This review explores the mechanisms of RNA-nano complexes-mediated plant protection, addressing RNA stability and nano-toxicity issues while examining the prospects of RNA-nano complex research in plant pathogen management.
在过去几十年中，RNA干扰（RNAi）已成为对抗致病真菌和卵菌的一种有效机制。它通过靶向参与由具有经济和科学重要性的真菌病原体（如 和 物种）引起的疾病的关键基因，提供了一种有前景的基因沉默方法。同时，纳米农产品作为植物保护策略中传统杀菌剂的替代品受到了关注。然而，细胞外环境中裸RNA分子的不稳定性带来了挑战，因为它们会迅速降解，限制了其长期疾病控制的功效。人们还对保护性纳米颗粒对非靶标生物的毒性表示担忧。将RNAi与纳米农产品，特别是纳米载体整合形成RNA-纳米复合物已显示出巨大潜力，可增强RNA稳定性、降低毒性并延长疾病控制时间。本综述探讨了RNA-纳米复合物介导的植物保护机制，解决RNA稳定性和纳米毒性问题，同时研究RNA-纳米复合物在植物病原体管理中的研究前景。
