Innovative PEGylated chitosan nanocarriers for co-delivery of doxorubicin and CpG in breast cancer therapy: Preparation, characterization, and immunotherapeutic potential.

This study aimed to design polyethylene glycol (PEG)ylated chitosan (CS, PEG-CS) nanoparticles for the co-delivery of doxorubicin (DOX), cytosine-phosphate-guanine oligodeoxynucleotide (CpG), and ovalbumin (OVA) to enhance breast cancer therapy. PEG-CS nanoparticles were synthesized using the ionotropic gelation method and characterized for size, zeta potential (ZP), entrapment efficiency, and drug release. In vitro and in vivo studies were conducted to assess cytotoxicity, immune activation, and antitumor efficacy. The optimized nanoparticles had a mean diameter of 156.4 ± 8.9 nm, a ZP of +18 mV, and demonstrated 75.3% DOX and 68.3% CpG release over 72 h. PEG-CS-DOX/CpG/OVA enhanced tumor reduction by 2.6-fold in vivo, with no significant toxicity. PEG-CS-DOX/CpG/OVA nanoparticles showed promise as a co-delivery platform for cancer therapy, combining cytotoxic and immune-stimulating effects with minimal toxicity.