BioEngineering Program, Georgia Institute of Technology, USA.
BioEngineering Program, Georgia Institute of Technology, USA; School of Chemical and Biomolecular Engineering, Georgia Institute of Technology, USA.
Biomaterials. 2024 Dec;311:122666. doi: 10.1016/j.biomaterials.2024.122666. Epub 2024 Jun 12.
Self-assembling protein nanoparticles are beneficial platforms for enhancing the often weak and short-lived immune responses elicited by subunit vaccines. Their benefits include multivalency, similar sizes as pathogens and control of antigen orientation. Previously, the design, preparation, and characterization of self-assembling protein vesicles presenting fluorescent proteins and enzymes on the outer vesicle surface have been reported. Here, a full-size model antigen protein, ovalbumin (OVA), was genetically fused to the recombinant vesicle building blocks and incorporated into protein vesicles via self-assembly. Characterization of OVA protein vesicles showed room temperature stability and tunable size. Immunization of mice with OVA protein vesicles induced strong antigen-specific humoral and cellular immune responses. This work demonstrates the potential of protein vesicles as a modular platform for delivering full-size antigen proteins that can be extended to pathogen antigens to induce antigen specific immune responses.
自组装蛋白纳米颗粒是增强亚单位疫苗引起的通常较弱和短暂免疫反应的有益平台。它们的优点包括多价性、与病原体相似的大小和抗原定向控制。此前，已经报道了在外壳表面呈现荧光蛋白和酶的自组装蛋白囊泡的设计、制备和表征。在这里，全长模型抗原蛋白卵清蛋白（OVA）被基因融合到重组囊泡构建块中，并通过自组装掺入蛋白囊泡中。OVA 蛋白囊泡的特性表明其在室温下稳定且尺寸可调。用 OVA 蛋白囊泡免疫小鼠可诱导强烈的抗原特异性体液和细胞免疫反应。这项工作证明了蛋白囊泡作为递送全长抗原蛋白的模块化平台的潜力，该平台可以扩展到病原体抗原以诱导抗原特异性免疫反应。
