Mediates the Tolerance to Abamectin and Beta-Cypermethrin in the Fall Armyworm .

Glutathione S-transferase (GST) is a class of detoxifying enzymes in the second stage of insect metabolism and plays a key role in insecticide resistance. In this study, based on the transcriptome sequences of , the full-length cDNA of was cloned and characterized. The temporal and spatial expression pattern showed that was highly expressed in Malpighian tubules, which are key excretion organs. Knocking down reduced tolerance to abamectin and beta-cypermethrin. The overexpression of enhanced the viability of cell under abamectin and beta-cypermethrin treatment. Furthermore, was overexpressed in using the / binary expression system, and this overexpression strain was also less susceptible to abamectin and beta-cypermethrin. The enzyme activity of recombinant SfGSTD1 could also be significantly inhibited by abamectin and beta-cypermethrin. Taken together, our findings indicate that might be involved in the tolerance of abamectin and beta-cypermethrin in . And these results provide theoretical foundations for understanding the resistance mechanism of to abamectin and beta-cypermethrin.