Zhang Zhifei, Su Xin, Shuo Dun, Yan Dawei, Pan Xue, Xu Bangfeng, Yan Minghao, Ren Shuxuan, Liu Qinfang, Yuan Chunxiu, Teng Qiaoyang, Li Zejun
Department of Avian Infectious Diseases, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, China.
Vet Sci. 2025 Mar 3;12(3):241. doi: 10.3390/vetsci12030241.
Duck egg-reducing syndrome virus (DERSV) is a novel Avihepatovirus and is responsible for a gradual decline in the laying rate of ducks, decreasing from a peak of 90% to 50%. The development of a rapid detection method for DERSV is crucial for the identification and control of virus infections. In this study, we developed a quantitative reverse transcription PCR (RT-qPCR) assay for detecting DERSV. Specific primers and a probe were designed to target a conserved region of the 3D gene. The assay demonstrated high specificity, with no cross-reactivity to other non-target duck viruses. It had a detection limit of 10 copies and a linear range from 10 to 10 copies per reaction. The assay's efficiency was 92.59%, with a regression coefficient (R) of 0.999. The coefficient of variation for both intra-and inter-assays was less than 2.00%. Among the 153 clinical samples collected from 2016 to 2023, the RT-qPCR detected a DERSV positive ratio of 47.06% (72/153). In conclusion, the utilization of the real-time RT-qPCR assay holds potential for the detection of DERSV in epidemiological and pathogenesis studies.
鸭减蛋综合征病毒（DERSV）是一种新型禽肝病毒，可导致鸭产蛋率逐渐下降，从峰值90%降至50%。开发一种快速检测DERSV的方法对于病毒感染的鉴定和控制至关重要。在本研究中，我们开发了一种用于检测DERSV的定量逆转录PCR（RT-qPCR）检测方法。设计了特异性引物和探针，靶向3D基因的保守区域。该检测方法具有高度特异性，与其他非目标鸭病毒无交叉反应。其检测限为10个拷贝，每个反应的线性范围为10至10拷贝。该检测方法的效率为92.59%，回归系数（R）为0.999。批内和批间变异系数均小于2.00%。在2016年至2023年收集的153份临床样本中，RT-qPCR检测到DERSV阳性率为47.06%（72/153）。总之，实时RT-qPCR检测方法在流行病学和发病机制研究中检测DERSV具有潜力。
