Ylä-Herttuala S, Nikkari T
Atherosclerosis. 1985 Jul;56(1):1-10. doi: 10.1016/0021-9150(85)90079-6.
The effects of post-mortem autolysis on the biochemical composition of coronary intima-medias were studied by keeping the coronary arteries of 25 slaughterhouse pigs at +4 degrees C for periods up to 7 days. The samples were analysed for DNA, total protein, collagen, glycosaminoglycans (GAGs), free and esterified cholesterol, triglycerides, phospholipid fractions, and fatty acid composition in various lipid classes. The content of sulphated GAGs decreased steadily, whereas that of hyaluronic acid remained unchanged. These alterations led to a significant decrease in the content of total GAGs and to an increase in the percentage of hyaluronic acid in total GAGs. The results indicate a continuous post-mortem degradation of arterial proteoglycans. After 7 days storage, there was a significant increase in the content of free fatty acids, and the composition of this fraction was changed by increases in the relative amounts of oleic and linoleic acids. These changes were accompanied by an increase in lysolecithin and a decrease in lecithin, which suggests a post-mortem action of arterial phospholipases.
通过将25头屠宰场猪的冠状动脉在4℃下保存长达7天，研究了死后自溶对冠状动脉内膜-中膜生化组成的影响。对样本进行了DNA、总蛋白、胶原蛋白、糖胺聚糖（GAGs）、游离胆固醇和酯化胆固醇、甘油三酯、磷脂组分以及各种脂质类别的脂肪酸组成分析。硫酸化GAGs的含量稳步下降，而透明质酸的含量保持不变。这些变化导致总GAGs含量显著下降，透明质酸在总GAGs中的百分比增加。结果表明动脉蛋白聚糖存在死后持续降解。储存7天后，游离脂肪酸含量显著增加，该组分的组成因油酸和亚油酸相对含量增加而改变。这些变化伴随着溶血卵磷脂增加和卵磷脂减少，这表明存在动脉磷脂酶的死后作用。
