Gjesdal K, Pepper D S
Thromb Haemost. 1977 Feb 28;37(1):73-80.
Human platelet factor 4 (PF-4) showed a reaction of complete identity with PF-4 from Macaca mulatta when tested against rabbit anti-human-PF-4. Such immunoglobulin was used for quantitative precipitation of in vivo labelled PF-4 in monkey serum. The results suggest that the active protein had an intra-platelet half-life of about 21 hours. In vitro 125I-labelled human PF-4 was injected intravenously into two monkeys and isolated by immuno-precipitation from platelet-poor plasma and from platelets disrupted after gel-filtration. Plasma PF-4 was found to have a half-life of 7 to 11 hours. Some of the labelled PF-4 was associated with platelets and this fraction had a rapid initial disappearance rate and a subsequent half-life close to that of plasma PF-4. The results are compatible with the hypothesis that granular PF-4 belongs to a separate compartment, whereas membrane-bound PF-4 and plasma PF-4 may interchange.
当用人血小板因子4（PF-4）与兔抗人PF-4进行检测时，其与猕猴的PF-4呈现完全相同的反应。这种免疫球蛋白用于定量沉淀猴血清中体内标记的PF-4。结果表明，活性蛋白在血小板内的半衰期约为21小时。将体外125I标记的人PF-4静脉注射到两只猴子体内，并通过免疫沉淀从贫血小板血浆和凝胶过滤后破坏的血小板中分离出来。发现血浆PF-4的半衰期为7至11小时。一些标记的PF-4与血小板相关，该部分最初的消失速率很快，随后的半衰期与血浆PF-4相近。这些结果与以下假设相符：颗粒状PF-4属于一个独立的区室，而膜结合PF-4和血浆PF-4可能会相互交换。
