Challacombe S J, Russell M W
Immunology. 1979 Feb;36(2):331-8.
Rhesus monkey IgG, IgA and IgM were purified from pooled normal serum and labelled with 125I. Four monkeys were injected intravenously with approximately 50 microgram of each purified immunoglobulin containing between 25 muCi and 50 muCi of 125I. Iodination was approximately 1 atom per 14 molecules of IgG and IgA and per 2.5 molecules of IgM. Samples of serum were taken for up to 22 days after injection and radioactivity compared with that in a sample taken within 30 min of injection. Sucrose density ultracentrifugation of serum samples revealed that in animals given IgG or IgA, radioactivity was associated with 7S protein peaks, and in animals given IgM radioactivity was associated predominantly with a 19S protein peak. Most of the radioactivity could be precipitated from serum samples with the appropriate monospecific antiserum. The mean half-life of rhesus monkey IgG was estimated as 8.3 days, IgA as 4.5 days and IgM as 4.7 days.
从混合的正常血清中纯化恒河猴IgG、IgA和IgM，并使用125I进行标记。给4只猴子静脉注射约50微克每种纯化的免疫球蛋白，其中含有25微居里至50微居里的125I。碘化程度约为每14个IgG和IgA分子以及每2.5个IgM分子中有1个原子。注射后长达22天采集血清样本，并将放射性与注射后30分钟内采集的样本中的放射性进行比较。血清样本的蔗糖密度超速离心显示，给予IgG或IgA的动物中，放射性与7S蛋白峰相关，给予IgM的动物中，放射性主要与19S蛋白峰相关。大多数放射性可通过适当的单特异性抗血清从血清样本中沉淀出来。恒河猴IgG的平均半衰期估计为8.3天，IgA为4.5天，IgM为4.7天。
