Su Shilong, Bai Jinwu, Wang Ruideng, Gao Shan, Zhou Rubing, Zhou Fang
Department of Orthopedics, Peking University Third Hospital, No.49 North Garden Road, Haidian, 100191, Beijing, China.
Engineering Research Center of Bone and Joint Precision Medicine, Peking University Third Hospital, No.49 North Garden Road, Haidian, 100191, Beijing, China.
Mater Today Bio. 2025 Apr 10;32:101759. doi: 10.1016/j.mtbio.2025.101759. eCollection 2025 Jun.
Patients with bone defects often have weak cell vitality and differentiation ability of endogenous bone marrow mesenchymal stem cells (BMSCs), which makes bone regeneration face challenges. At present, the bone tissue engineering strategies are mainly to build grafts by loading cells on scaffolds in vitro. These strategies face many difficulties that limit their clinical application. To this end, we developed a new strategy for bone defect repair, namely chemotactic cell-free scaffolds combined with BMSCs injection. We first prepared a polydopamine-functionalized acellular fish scale scaffold that can continuously release stromal cell-derived factor 1α (SDF-1α) (termed as SDF-1α/PAFS) in vivo for at least 10 days. The study results showed that the scaffold not only has excellent mechanical properties and good biocompatibility but also has reactive oxygen scavenging activity, immunomodulation, angiogenesis, and osteogenesis. More importantly, SDF-1α/PAFS can recruit postoperatively injected BMSCs into bone defects for bone repair. We constructed the mouse cranial bone defect model, and in vivo experimental results confirmed that the strategy of combining SDF-1α/PAFS with BMSCs injection can effectively promote bone defect repair. Overall, this study provides a promising strategy for bone defect repair, with better clinical convenience and operability.
骨缺损患者的内源性骨髓间充质干细胞（BMSCs）通常具有较弱的细胞活力和分化能力，这使得骨再生面临挑战。目前，骨组织工程策略主要是通过在体外将细胞加载到支架上来构建移植物。这些策略面临许多限制其临床应用的困难。为此，我们开发了一种新的骨缺损修复策略，即趋化性无细胞支架联合BMSCs注射。我们首先制备了一种聚多巴胺功能化的无细胞鱼鳞支架，该支架能够在体内持续释放基质细胞衍生因子1α（SDF-1α）（称为SDF-1α/PAFS）至少10天。研究结果表明，该支架不仅具有优异的力学性能和良好的生物相容性，还具有活性氧清除活性、免疫调节、血管生成和成骨作用。更重要的是，SDF-1α/PAFS能够将术后注射的BMSCs募集到骨缺损处进行骨修复。我们构建了小鼠颅骨缺损模型，体内实验结果证实，SDF-1α/PAFS联合BMSCs注射的策略能够有效促进骨缺损修复。总体而言，本研究为骨缺损修复提供了一种有前景的策略，具有更好的临床便利性和可操作性。
