Su Shilong, Wang Ruideng, Bai Jinwu, Chen Zhengyang, Zhou Fang
Department of Orthopedics, Peking University Third Hospital, No. 49 North Garden Road, Haidian, 100191 Beijing, China.
Engineering Research Center of Bone and Joint Precision Medicine, Peking University Third Hospital, No. 49 North Garden Road, Haidian, 100191 Beijing, China.
ACS Omega. 2025 Jan 1;10(1):230-238. doi: 10.1021/acsomega.4c05096. eCollection 2025 Jan 14.
In bone tissue engineering, a suitable scaffold is the key. Due to their similar composition to bone tissue, special structure, good mechanical properties, and osteogenic properties, acellular fish scale scaffolds are potential scaffolds for bone tissue engineering. At present, the fish scale decellularization scheme mostly uses a combination of sodium dodecyl sulfate and ethylenediamine tetraacetic acid (EDTA), but this method has problems. We optimized this method using a combined method of Triton X-100, EDTA, and nuclease. In this study, the optimal scheme was screened with respect to the decellularization effect, extracellular matrix composition and structure retention, mechanical properties, cell biocompatibility, and osteogenic differentiation ability. The results showed that the optimal scheme was as follows: the native fish scales were incubated in 0.1% EDTA for 24 h, and then the cellular components were removed with 1% Triton X-100 for 4 days, followed by nuclease digestion for 24 h. On that basis, we proposed a novel and more suitable fish scale decellularization scheme, and the acellular fish scale scaffold prepared by this decellularization scheme may have great potential in bone tissue engineering.
在骨组织工程中，合适的支架是关键。由于其与骨组织组成相似、结构特殊、力学性能良好以及具有成骨特性，脱细胞鱼鳞支架是骨组织工程的潜在支架。目前，鱼鳞脱细胞方案大多采用十二烷基硫酸钠和乙二胺四乙酸（EDTA）联合使用的方法，但该方法存在问题。我们采用Triton X - 100、EDTA和核酸酶联合的方法对该方法进行了优化。在本研究中，从脱细胞效果、细胞外基质组成和结构保留、力学性能、细胞生物相容性和成骨分化能力等方面筛选出了最佳方案。结果表明，最佳方案如下：将天然鱼鳞在0.1% EDTA中孵育24小时，然后用1% Triton X - 100去除细胞成分4天，接着进行核酸酶消化24小时。在此基础上，我们提出了一种新颖且更合适的鱼鳞脱细胞方案，通过该脱细胞方案制备的脱细胞鱼鳞支架在骨组织工程中可能具有巨大潜力。
