Bian Tingting, Zheng Miaoseng, Wang Ting, Zhang Qing, Zhang Jianguo, Liu Yifei, Shi Wenyu
Suzhou Medical College of Soochow University, Suzhou, Jiangsu 215006, P.R. China.
Department of Pathology, Affiliated Hospital of Nantong University, Nantong, Jiangsu 226001, P.R. China.
Oncol Lett. 2025 Apr 11;29(6):292. doi: 10.3892/ol.2025.15038. eCollection 2025 Jun.
The expression levels of DEAD-box 46 (DDX46) are elevated in several malignancies; however, the function of DDX46 in lung adenocarcinoma (LUAD), including its expression patterns and functional implications, has not been fully elucidated. The present study primarily explores the potential role and underlying mechanism of DDX46 in the malignant progression of LUAD. The present study analyzed both publicly available databases and clinical specimens to assess DDX46 expression in LUAD and explore its prognostic significance. The findings demonstrated that elevated DDX46 expression was associated with a worse prognosis in patients with LUAD in comparison with a low DDX46 expression. Functional assays, including Cell Counting Kit-8, colony formation, 5-ethynyl-2'-deoxyuridine incorporation, flow cytometry, wound healing and Transwell assays, indicated that silencing DDX46 suppressed cancer cell migration, enhanced apoptosis, and induced G/G phase cell cycle arrest. Moreover, DDX46 expression was correlated with the infiltration of T cells, natural killer cells and monocytes, as well as with several immune checkpoints and chemokines. Additionally, the results identified a marked association between DDX46 and the Wnt signaling pathway in LUAD. Low DDX46 expression was also demonstrated to be associated with increased drug responsiveness in patients. In conclusion, DDX46 holds promise as a dual-purpose marker for the diagnosis and therapy of patients with LUAD.
DEAD盒蛋白46（DDX46）在多种恶性肿瘤中表达水平升高；然而，DDX46在肺腺癌（LUAD）中的功能，包括其表达模式和功能意义，尚未完全阐明。本研究主要探讨DDX46在LUAD恶性进展中的潜在作用及潜在机制。本研究分析了公开可用的数据库和临床标本，以评估DDX46在LUAD中的表达，并探讨其预后意义。研究结果表明，与低DDX46表达相比，LUAD患者中DDX46表达升高与较差的预后相关。功能分析，包括细胞计数试剂盒-8、集落形成、5-乙炔基-2'-脱氧尿苷掺入、流式细胞术、伤口愈合和Transwell分析，表明沉默DDX46可抑制癌细胞迁移、增强细胞凋亡并诱导G/G期细胞周期阻滞。此外，DDX46表达与T细胞、自然杀伤细胞和单核细胞的浸润以及几种免疫检查点和趋化因子相关。此外，结果确定了DDX46与LUAD中Wnt信号通路之间存在显著关联。低DDX46表达也被证明与患者药物反应性增加有关。总之，DDX有望成为LUAD患者诊断和治疗的双重用途标志物。
