Bell S C, Hales M W, Patel S, Kirwan P H, Drife J O
Br J Obstet Gynaecol. 1985 Aug;92(8):793-803. doi: 10.1111/j.1471-0528.1985.tb03048.x.
To investigate the role of the endometrium in sustaining early pregnancy, specimens of endometrium from early pregnancy were incubated in vitro with radiolabelled amino acids, and protein synthesis and secretion were studied by subsequent fluorographic analysis of one-and two-dimensional polyacrylamide gels. Seventeen secreted endometrial proteins (EP) were identified. Changes were demonstrated in the rate of synthesis and/or secretion of a number of these proteins during early pregnancy. Two proteins, EP 14 and EP 15, are the principal secretory proteins of pregnancy endometrium. EP 14 (subunit mol. wt 32 000) is associated with decidualized endometrium and its rate of synthesis/secretion increases during early pregnancy. EP 15 (subunit mol. wt 28 000) is also synthesized and secreted by the secretory endometrium during the menstrual cycle, and during early pregnancy, but its secretion declines to undetectable levels by week 15-16. These proteins may provide useful markers of endometrial function and differentiation during the menstrual cycle and pregnancy.
为研究子宫内膜在维持早期妊娠中的作用，将早孕子宫内膜标本与放射性标记氨基酸进行体外孵育，随后通过对一维及二维聚丙烯酰胺凝胶进行荧光显影分析来研究蛋白质的合成与分泌。鉴定出17种分泌型子宫内膜蛋白（EP）。在早孕期间，其中多种蛋白质的合成和/或分泌速率出现了变化。两种蛋白，EP 14和EP 15，是妊娠子宫内膜的主要分泌蛋白。EP 14（亚基分子量32 000）与蜕膜化子宫内膜相关，其合成/分泌速率在早孕期间增加。EP 15（亚基分子量28 000）在月经周期及早孕期间也由分泌期子宫内膜合成并分泌，但其分泌在第15 - 16周时降至检测不到的水平。这些蛋白质可能为月经周期和妊娠期间子宫内膜功能及分化提供有用的标志物。
