This study evaluated the effectiveness of antibiotic, probiotic, and antimicrobial peptide (AMP) supplements in mitigating adverse consequences of necrotic enteritis (NE) in broilers. In total, 720 one-day-old (Ross-308) male broiler chicks were randomly assigned to five distinct feeding regimens (each treatment consisting of six replicates of 24 birds) including: (1) negative control (NC), fed only basal diet; (2) positive control (PC); with C. perfringens challenge + basal diet; (3) CP-Ab: challenged + virginiamycin (Stafac® 500) at 200 g/ton, (4) CP-Pro: challenged + 200 g/ton B. subtilis PB6 (Clostat dry®) probiotic additive, and (5) CP-LS2: challenged and fed an antimicrobial peptide (LassoTide Plus®) at 200 g/ton. The NE challenge was induced by administering 10X coccidia vaccine on day 15 followed by inoculation with a pathogenic field strain of C. perfringens type G (1 × 10 CFU/ml/ bird; 1 ml) on days 19 and 20 through oral gavage. Feeding AMP and probiotic to the NE-affected broilers resulted in 23.93% and 19.70% respectively higher body weight gain and 76.59% and 70.27% lower mortality compared to the PC. Similarly, supplementation with AMP improved (P < 0.05) gut morphology, carcass yield (5.82%), meat water holding capacity (14.73%), and reduced cooking loss (10.01%), dripping loss (20.87%), and shear force (25%) as compared to the PC. Additionally, the excreta score, litter quality and welfare attributes were significantly ameliorated (P < 0.05) with AMP addition. In conclusion, the findings suggest that AMP outperformed both virginiamycin and probiotic, demonstrating its potential as a superior substitute for AGPs in broilers.