用于空间转录组学的新鲜冷冻大豆组织制备方案。
Protocol for preparing fresh frozen soybean tissues for spatial transcriptomics.
作者信息
Luo Ziliang, Schmitz Robert J
机构信息
Department of Genetics, University of Georgia, Athens, GA, USA.
Department of Genetics, University of Georgia, Athens, GA, USA.
出版信息
STAR Protoc. 2025 Apr 23;6(2):103790. doi: 10.1016/j.xpro.2025.103790.
Spatial transcriptomics is a disruptive technology that enables the identification of cell-type-specific transcripts en masse. However, the use of spatial transcriptomics for plant tissues has been challenging due to issues related to tissue preparation. Here, we present a protocol for preparing fresh frozen soybean tissues for spatial transcriptomics. We describe the steps for embedding, cryosectioning, fixation, staining, and imaging of soybean tissues. We then detail procedures for library preparation and sequencing. For complete details on the use and execution of this protocol, please refer to Zhang et al..
空间转录组学是一项突破性技术，能够大规模鉴定细胞类型特异性转录本。然而，由于与组织制备相关的问题，将空间转录组学应用于植物组织一直具有挑战性。在这里，我们提出了一种用于为空间转录组学制备新鲜冷冻大豆组织的方案。我们描述了大豆组织包埋、冷冻切片、固定、染色和成像的步骤。然后，我们详细介绍了文库制备和测序的程序。有关本方案的使用和执行的完整详细信息，请参考Zhang等人的文献。
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