拟南芥全基因组染色质相互作用捕获方案。

Protocol for capturing genome-wide chromatin interactions in Arabidopsis.

作者信息

Shu Jiayue, Xiao Suxin, Wang Dingyue, Yang Minqi, Zhou Yue

机构信息

State Key Laboratory of Gene Function and Modulation Research, School of Advanced Agricultural Sciences, Peking-Tsinghua Center for Life Sciences, Peking University, Beijing 100871, China.

出版信息

STAR Protoc. 2025 Mar 16;6(2):103702. doi: 10.1016/j.xpro.2025.103702.

DOI:10.1016/j.xpro.2025.103702

PMID:40096089

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11957576/

Abstract

High-order chromatin structure is essential for the regulation of plant growth and development. Here, we present a protocol for capturing genome-wide chromatin interactions in Arabidopsis. We describe steps for tissue fixation, nuclei isolation, chromatin digestion, ligation, sonication, and library preparation. This protocol has potential application in 3D chromatin analysis in many species of plants. For complete details on the use and execution of this protocol, please refer to Shu et al., Yin et al., and Yang et al..

摘要

高阶染色质结构对于植物生长发育的调控至关重要。在此，我们展示了一种用于捕获拟南芥全基因组染色质相互作用的方案。我们描述了组织固定、细胞核分离、染色质消化、连接、超声处理和文库制备的步骤。该方案在许多植物物种的三维染色质分析中具有潜在应用。有关该方案使用和执行的完整详细信息，请参考舒等人、尹等人和杨等人的研究。