Villalobos-Gómez Fabiola Del Rocío, Palacios-Marmolejo Anastacio, Salinas-Gutiérrez Rogelio, Lagunes-Servín Hugo Enrique
Instituto Mexicano del Seguro Social, Hospital General de Zona No. 3, Servicio de Patología. Aguascalientes, Aguascalientes, México.
Instituto de Salud del Estado de Aguascalientes, Laboratorio Estatal de Aguascalientes, Laboratorio de Biología Molecular. Aguascalientes, Aguascalientes, México.
Rev Med Inst Mex Seguro Soc. 2025 Mar 3;63(2):e6353. doi: 10.5281/zenodo.14617070.
SARS-CoV-2 is associated with the activation of innate immunity, with an observed increase in neutrophils, mononuclear phagocytes, and natural killer cells, as well as a decrease in T cells. Interferon plays important roles in restricting viruses and is crucial because it participates in both innate and adaptive immunity. Gamma interferon (IFN-γ) is a cytokine that plays a fundamental role in maintaining homeostasis in the body, both in vitro and in vivo.
An immunological study was conducted to measure the concentrations of IFN-γ in severe SARS-CoV-2-positive patients.
Around 500 sera from patients with similar respiratory comorbidities at the onset of the disease were analyzed, using healthy patients as controls. The sera from SARS-CoV-2-positive individuals were processed using the ELISA technique, and the statistical treatment of the data involved creating a database with the absorbance readings, which were then converted into concentrations in pg/ml.
We can infer that the amount of IFN-γ expression depends on the patient's condition; the more severe the patient's condition, the higher the expression of this cytokine compared to their baseline levels, ranging from 0 to 0.5 μg/ml.
It is suggested that the IFN-γ response plays an important role in controlling the disease, and effective therapies are recommended for the treatment of Covid-19, which will lead to the implementation of therapeutic approaches aimed at counteracting the immune system, especially in the more severe forms of the disease.
严重急性呼吸综合征冠状病毒2（SARS-CoV-2）与固有免疫激活相关，表现为中性粒细胞、单核吞噬细胞和自然杀伤细胞数量增加，以及T细胞数量减少。干扰素在限制病毒方面发挥重要作用，且至关重要，因为它参与固有免疫和适应性免疫。γ干扰素（IFN-γ）是一种细胞因子，在体内外维持机体稳态中发挥着重要作用。
进行一项免疫学研究，以测量重症SARS-CoV-2阳性患者体内IFN-γ的浓度。
以健康患者作为对照，分析了约500例疾病发作时具有相似呼吸道合并症患者的血清。采用酶联免疫吸附测定（ELISA）技术处理SARS-CoV-2阳性个体的血清，数据的统计处理包括创建一个包含吸光度读数的数据库，然后将其转换为pg/ml浓度。
我们可以推断，IFN-γ的表达量取决于患者的病情；患者病情越严重，与基线水平（0至0.5μg/ml）相比，这种细胞因子的表达越高。
提示IFN-γ反应在控制疾病中发挥重要作用，建议采用有效的治疗方法治疗新型冠状病毒肺炎（Covid-19），这将促使实施旨在对抗免疫系统的治疗方法，尤其是针对病情更严重的患者。
