McKay D J, Renaux B S, Dixon G H
Biosci Rep. 1985 May;5(5):383-91. doi: 10.1007/BF01116555.
Human sperm protamines have been extracted from spermatozoa pooled from several donors, converted to their S-pyridylethylated derivatives and resolved into two major components, P1 and P2, by Bio-Rex 70 chromatography. Protamine P1 was further purified by Bio-Gel P-10 chromatography and sequenced directly on a gas phase protein sequencer for 43 residues. To complete the sequence, P1 was cleaved at methionine 36 and the C-terminal tetradecapeptide was purified by h.p.l.c. and sequenced completely. The 50 residue sequence is: (sequence see text) This sequence has a calculated molecular weight of 6674 and is homologous with four other published mammalian protamine sequences.
已从多个供体的精液中提取人精子鱼精蛋白，将其转化为S-吡啶基乙基化衍生物，并通过Bio-Rex 70色谱法分离为两个主要成分P1和P2。鱼精蛋白P1通过Bio-Gel P-10色谱法进一步纯化，并在气相蛋白质测序仪上直接对43个残基进行测序。为完成序列测定，在甲硫氨酸36处切割P1，通过高效液相色谱法纯化C末端十四肽并进行完全测序。50个残基的序列为：（序列见正文）该序列的计算分子量为6674，与其他四个已发表的哺乳动物鱼精蛋白序列同源。
