Emson P C, Corder R, Ratter S J, Tomlin S, Lowry P J, Ress L H, Arregui A, Rosser M N
Neuroendocrinology. 1984 Jan;38(1):45-50. doi: 10.1159/000123864.
The regional distribution of pro-opiocortin-derived peptides and methionine enkephalin was investigated in human brain post-mortem. Sequence-directed radioimmunoassays for beta-endorphin, gamma-lipotropin, adrenocorticotrophin, corticotrophin-like intermediate lobe peptide (ACTH18-39, CLIP) alpha-MSH and methionine enkephalin were used and 40 different human brain areas were assayed. The regional distribution of all the pro-opiomelanocortin-derived immunoreactivities were correlated with highest amounts of beta-endorphin, gamma-lipotropin and ACTH in the hypothalamus, amygdala, periventricular grey, substantia nigra and superior colliculus. The distribution of beta-endorphin, gamma-lipotropin and ACTH did not parallel the distribution of methionine-enkephalin immunoreactivity which was present in the globus pallidus, nucleus accumbens and substantia nigra. Gel exclusion chromatography (G-50) showed that pro-opiocortin-related peptides in the human hypothalamus and periventricular grey separated in positions consistent with the major immunoreactive forms being beta-endorphin, gamma-lipotropin, ACTH and CLIP.
对人死后大脑中阿片促皮质素原衍生肽和甲硫氨酸脑啡肽的区域分布进行了研究。采用针对β-内啡肽、γ-促脂素、促肾上腺皮质激素、促肾上腺皮质激素样中叶肽（ACTH18 - 39，CLIP）、α-促黑素细胞激素和甲硫氨酸脑啡肽的序列导向放射免疫分析法，对40个不同的人脑区域进行了检测。所有阿片促黑素原衍生免疫反应性的区域分布与下丘脑、杏仁核、室周灰质、黑质和上丘中β-内啡肽、γ-促脂素和促肾上腺皮质激素的最高含量相关。β-内啡肽、γ-促脂素和促肾上腺皮质激素的分布与甲硫氨酸脑啡肽免疫反应性的分布并不平行，后者存在于苍白球、伏隔核和黑质中。凝胶过滤色谱法（G - 50）显示，人下丘脑和室周灰质中与阿片促皮质素原相关的肽在与主要免疫反应形式为β-内啡肽、γ-促脂素、促肾上腺皮质激素和CLIP一致的位置分离。
